Reconstitution of Human Keloids in Mouse Skin

Plastic and Reconstructive Surgery. Global Open
Ataru SunagaKotaro Yoshimura

Abstract

Keloids are a dermal fibroproliferative scar of unknown etiology. There is no good animal model for the study of keloids, which hinders the development and assessment of treatments for keloids. Human keratinocytes and dermal fibroblasts were isolated from 3 human skin tissues: normal skin, white scars, and keloids. A mixed-cell slurry containing keratinocytes and dermal fibroblasts was poured into a double chamber implanted on the back of NOD/Shi-scid/IL-2Rγnull mice. After 12 weeks, the recipient mice had developed reconstituted human skin tissues on their backs. These were harvested for histological studies. Macroscopically, the reconstituted skins derived from both normal skin and white scars were similar to normal skin and white scars in humans, respectively. Keloid-derived reconstituted skins exhibited keloid-like hypertrophic nodules. Histological findings and immunohistochemical staining confirmed that the reconstituted skin tissues were of human origin and the keloid-derived reconstituted skin had the typical features of human keloids such as a hypertrophic dermal nodule, collagen type composition, orientation of collagen fibers, and versican expression. The mouse model with humanized keloid tissue presented here should...Continue Reading

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Citations

Feb 15, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Maribanyana LebekoArdeshir Bayat
May 24, 2019·Advances in Wound Care·Tae Hwan ParkYuri Cho
Feb 23, 2020·Stem Cell Research & Therapy·Subramanian ArjunanChui Yee Fong
Dec 14, 2019·Advances in Wound Care·Dorothy M Supp
Mar 6, 2021·The Laryngoscope·Ellen M HongBrian J F Wong
Jun 18, 2021·The FEBS Journal·Donovan Correa-Gallegos, Yuval Rinkevich
Jun 22, 2021·Experimental Dermatology·Andrew W StevensonMark W Fear

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Methods Mentioned

BETA
polarization microscopy
transgenic
xenografts

Software Mentioned

ImageJ

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