Reconstitution of mouse spermatogonial stem cell niches in culture

Cell Stem Cell
Mito Kanatsu-ShinoharaTakashi Shinohara

Abstract

Spermatogonial stem cells (SSCs) reside in specific niches within seminiferous tubules. These niches are thought to secrete chemotactic factors for SSCs, because SSCs migrate to them upon transplantation. However, the identity of these chemotactic molecules remains unknown. Here, we established a testis feeder cell culture system and used it to identify SSC chemotactic factors. When seeded on testis cells from infertile mice, SSCs migrated beneath the Sertoli cells and formed colonies with a cobblestone appearance that were very similar to those produced by hematopoietic stem cells. Cultured cells maintained SSC activity and fertility for at least 5 months. Cobblestone colony formation depended on GDNF and CXCL12, and dominant-negative GDNF receptor transfection or CXCL12 receptor deficiency reduced SSC colonization. Moreover, GDNF upregulated CXCL12 receptor expression, and CXCL12 transfection in Sertoli cells increased homing efficiency. Overall, our findings identify GDNF and CXCL12 as SSC chemotactic factors in vitro and in vivo.

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Jul 1, 1982·Journal of Cellular Physiology·H A KodamaS Kasai
Dec 3, 1993·Cell·M RassoulzadeganF Cuzin
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, J W Zimmermann
Jan 14, 1998·Nature Medicine·G A DonzellaJ P Moore
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Mar 20, 2002·Mechanisms of Development·Yuko TadokoroYoshitake Nishimune
Apr 23, 2002·Biology of Reproduction·Takashi ShinoharaRalph L Brinster
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Masahito IkawaInder M Verma
Apr 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Toshiaki AraTakashi Nagasawa
Apr 18, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jun 29, 2005·Cytokine & Growth Factor Reviews·Elena ArighiHannu Sariola
Oct 21, 2005·Biology of Reproduction·Cathy K NaughtonJeffrey Milbrandt
Apr 13, 2007·Development·Jiyoung LeeTakashi Shinohara
Feb 13, 2008·Cell·Bijan BoldajipourErez Raz
Nov 6, 2008·Cell Stem Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Nov 19, 2010·Biology of Reproduction·Melissa J OatleyJon M Oatley

❮ Previous
Next ❯

Citations

Jan 15, 2013·Asian Journal of Andrology·Kate L Loveland, Eileen A McLaughlin
Jul 16, 2014·Molecular and Cellular Endocrinology·Dante Lamberti, Elena Vicini
Mar 14, 2013·BioMed Research International·Laura A Martin, Marco Seandel
Oct 9, 2013·Annual Review of Cell and Developmental Biology·Mito Kanatsu-Shinohara, Takashi Shinohara
Feb 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Liang-Yu ChenEdward M Eddy
Jan 14, 2016·International Journal of Molecular Sciences·Saishu YoshidaYukio Kato
Feb 6, 2016·Andrology·L R FrançaM D Griswold
Jan 19, 2016·Animal Reproduction Science·Huan CaiXue-Ming Zhang
Feb 24, 2016·Stem Cells International·Zhiyv NiuRalph L Brinster
Mar 10, 2016·Seminars in Cell & Developmental Biology·Carla BoitaniElena Vicini
Nov 22, 2013·The Journal of Reproduction and Development·Kei IshiiTakashi Shinohara
Mar 7, 2014·Seminars in Cell & Developmental Biology·Kin Lam FokHsiao Chang Chan
Dec 17, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Su-Ren Chen, Yi-Xun Liu
Jun 4, 2016·Biochemical and Biophysical Research Communications·Aya UchidaYoshiakira Kanai
Apr 14, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Hong WangZuping He
Nov 8, 2013·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Dec 19, 2017·Frontiers in Endocrinology·Kate L LovelandHans-Christian Schuppe
Feb 9, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Laura HeckmannNina Neuhaus
Sep 4, 2018·The Journal of Reproduction and Development·Mito Kanatsu-ShinoharaTakashi Shinohara
Sep 10, 2014·Journal of Assisted Reproduction and Genetics·Mohammad Sadra ShiraziMohammad Mehdi Akhondi
Mar 13, 2020·Human Reproduction Update·Murat GulEllen Goossens
Apr 7, 2020·Nature Biomedical Engineering·Emma S GargusTeresa K Woodruff
Aug 6, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Romain LambrotSarah Kimmins
Dec 28, 2017·Human Reproduction Update·João Pedro Alves-Lopes, Jan-Bernd Stukenborg
Sep 22, 2017·Primate Biology·Swati SharmaStefan Schlatt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.