Aug 1, 1992

Reconstitution of the brain mixed function oxidase system: purification of NADPH-cytochrome P450 reductase and partial purification of cytochrome P450 from whole rat brain

Journal of Neurochemistry
A F Bergh, H W Strobel

Abstract

NADPH-cytochrome P450 reductase was purified to apparent homogeneity and cytochrome P450 partially purified from whole rat brain. Purified reductase from brain was identical to liver P450 reductase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot techniques. Kinetic studies using cerebral P450 reductase reveal Km values in close agreement with those determined with enzyme purified from rat liver. Moreover, the brain P450 reductase was able to function successfully in a reconstituted microsomal system with partially purified brain cytochrome P450 and with purified hepatic P450c (P450IA1) as measured by 7-ethoxycoumarin and 7-ethoxyresorufin O-deethylation. Our results indicate that the reductase and P450 components may interact to form a competent drug metabolism system in brain tissue.

Mentioned in this Paper

NADPH-Ferrihemoprotein Reductase
Western Blotting
Cyp2c12
SDS-PAGE
August Rats
Cytochrome P450
Brain
Hepatic
Cyp1a1
Cytochrome P-450 Oxygenase

About this Paper

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