PMID: 7535851Oct 1, 1994Paper

Reconstitution of the influenza virus M2 ion channel in lipid bilayers

The Journal of Membrane Biology
M T TostesonRobert A Lamb

Abstract

M2, an integral membrane protein of influenza A virus, was purified from either influenza A virus-infected CV-1 cells or from Spodoptera frugiperda (Sf9) cells infected with a recombinant-M2 baculovirus. The purified protein, when incorporated into phospholipid bilayer membranes, produced ion-permeable channels with the following characteristics: (1) The channels appeared in bursts during which unit conductances of diverse magnitudes (25-500 pS) were observed. (2) The most probable open state was usually the lowest unit conductance (25-90 pS). (3) The channels were selective for cations; tNa = 0.75 when 150 mM NaCl bathed both sides of the membrane. (4) Amantadine reduced the probablity of opening of the high conductance state and also the conductance of the most probable state. (5) Reducing pH increased the mean current through the open channel as well as the conductance of the most probable state. (6) The sequence of selectivity for group IA monovalent cations was Rb > K > Cs approximately Na > Li. The pH activation, amantadine block and ion selectivity of the M2 protein ion channel in bilayers are consistent with those observed on expression of the M2 protein in oocytes of Xenopus laevis as well as for those predicted for th...Continue Reading

Citations

Feb 27, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M S SansomR Bull
Jul 12, 2005·The Journal of Membrane Biology·A PremkumarP W Gage
Jun 29, 2004·European Biophysics Journal : EBJ·Cornelia SchroederTse-I Lin
Apr 1, 1995·Antiviral Research·D W KimberlinR J Whitley
Aug 1, 1995·Current Opinion in Structural Biology·M Montal
Jan 8, 1999·Virus Research·Y TadaK Nakamura
Aug 1, 1997·Current Opinion in Structural Biology·G R Dieckmann, W F DeGrado
Jul 18, 2002·Seminars in Pediatric Infectious Diseases·Charles G Prober
Apr 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jun HuTimothy A Cross
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S C PillerP W Gage
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·T SakaguchiR A Lamb
Oct 14, 2004·European Journal of Biochemistry·Tosti J MankelowLydia M Henderson
Oct 20, 2007·Virology Journal·Joshua M Costin
Feb 26, 2013·Chemical Reviews·Lukas WankaPeter R Schreiner
Jan 26, 2016·Biochimica Et Biophysica Acta·Wolfgang B FischerDieter Heermann
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Changlin TianTimothy A Cross
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Lidia CristianWilliam F DeGrado
Mar 20, 2009·Proteins·Morad MustafaDavid D Busath
Sep 21, 2010·Cell·Jeremy S RossmanRobert A Lamb
Apr 1, 1997·FEBS Letters·A L GriceM S Sansom
Sep 23, 2010·Biophysical Journal·Sandra SchickKalina Hristova
Nov 6, 2004·Virology·Lauren WilsonGary Ewart
May 19, 2010·Biochimica Et Biophysica Acta·Kai WangBing Sun
Jul 26, 2005·Biophysical Journal·Yujie Wu, Gregory A Voth
Aug 16, 2016·Journal of Molecular Biology·Dror AssaIsaiah T Arkin
Nov 9, 2016·Protein Expression and Purification·Elodie Desuzinges MandonAnass Jawhari
Jul 8, 2015·Viruses·Jose L Nieto-TorresLuis Enjuanes
Sep 16, 2003·FEBS Letters·Marie L KellyLawrence H Pinto
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Lawrence H Pinto, Robert A Lamb
Jan 12, 2007·Molecular BioSystems·Lawrence H Pinto, Robert A Lamb
Mar 18, 2000·The Journal of Biological Chemistry·J A MouldL H Pinto
Apr 3, 1999·The Journal of Biological Chemistry·A J Adduci, R Schlegel
Feb 20, 1999·The Journal of Biological Chemistry·C S GandhiL H Pinto
Jul 27, 2000·The Journal of Biological Chemistry·J A MouldL H Pinto
Dec 15, 2000·The New England Journal of Medicine·R B Couch
Aug 17, 2002·The Journal of Biological Chemistry·Yajun TangLawrence H Pinto
Mar 29, 2003·Physiological Reviews·Thomas E Decoursey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.