Reconstructing Lineage Hierarchies of Mouse Uterus Epithelial Development Using Single-Cell Analysis

Stem Cell Reports
Bingbing WuXiaohui Zou

Abstract

The endometrial layer comprises luminal and glandular epithelia that both develop from the same simple layer of fetal uterine epithelium. Mechanisms of uterine epithelial progenitor self-renewal and differentiation are unclear. This study aims to systematically analyze the molecular and cellular mechanisms of uterine epithelial development by single-cell analysis. An integrated set of single-cell transcriptomic data of uterine epithelial progenitors and their differentiated progenies is provided. Additionally the unique molecular signatures of these cells, characterized by sequential upregulation of specific epigenetic and metabolic activities, and activation of unique signaling pathways and transcription factors, were also investigated. Finally a unique subpopulation of early progenitor, as well as differentiated luminal and glandular lineages, were identified. A complex cellular hierarchy of uterine epithelial development was thus delineated. Our study therefore systematically decoded molecular markers and a cellular program of uterine epithelial development that sheds light on uterine developmental biology.

References

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Citations

Aug 9, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Zer Vue, Richard R Behringer
Mar 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shiying Jin
Mar 16, 2019·Scientific Reports·Michael L MucenskiS Steven Potter
Jan 25, 2020·Science·Gunsagar S GulatiAaron M Newman
Feb 2, 2021·Science China. Life Sciences·Shaojun WangGuang-Hua Peng
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Vineet K MauryaJohn P Lydon

Datasets Mentioned

BETA
GSE98451
GSE98438

Methods Mentioned

BETA
RNA-Seq
flow-cytometry
CEL-Seq
acetylation
fluorescence-activated cell sorting
FACS
cDNA library construction
immunoprecipitation
PCR
in vitro transcription

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