Reconstruction of aperture functions during full fusion in vesicular exocytosis of neurotransmitters

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Christian AmatoreIrina Svir

Abstract

Individual vesicular exocytosis of adrenaline by dense core vesicles in chromaffin cells is considered here as a paradigm of many situations encountered in biology, nanosciences and drug delivery in which a spherical container releases in the external environment through gradual uncovering of its surface. A procedure for extracting the aperture (opening) function of a biological vesicle fusing with a cell membrane from the released molecular flux of neurotransmitter as monitored by amperometry has been devised based on semi-analytical expressions derived in a former work [C. Amatore, A. I. Oleinick, I. Svir, ChemPhysChem 2009, 10, DOI: 10.1002/cphc.200900646]. This precise analysis shows that in the absence of direct information about the radius of the vesicle or about the concentration of the adrenaline cation stored by the vesicle matrix, current spikes do not contain enough information to determine the maximum aperture angle. Yet, a statistical analysis establishes that this maximum aperture angle is most probably less than a few tens of degrees, which suggests that full fusion is a very improbable event.

References

Jun 1, 1977·The British Journal of Venereal Diseases·J B Robbins
Dec 11, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M WightmanO H Viveros
Aug 1, 1996·Current Opinion in Cell Biology·A Kusumi, Y Sako
Feb 29, 2000·Biophysical Journal·V NoireauxC Sykes
Jan 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ann-Sofie CansAndrew Ewing
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hideji MurakoshiAkihiro Kusumi
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Céline RevenuDaniel Louvard
Jan 11, 2005·In Vitro Cellular & Developmental Biology. Animal·Gertrude Case BuehringMichael J Eby
Jul 6, 2005·Trends in Neurosciences·Jakob B Sørensen
Aug 23, 2005·Brain Research. Brain Research Reviews·Carlos Fernández-PeruchenaGuillermo Alvarez de Toledo
Aug 25, 2005·Nature Methods·Eugene V Mosharov, David Sulzer
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Meyer B Jackson, Edwin R Chapman
Jun 30, 2006·Journal of Neurochemistry·Nobutoshi C HarataRichard W Tsien
Jul 25, 2007·Traffic·Silvio O Rizzoli, Reinhard Jahn
Mar 21, 2008·Physical Review Letters·Karin JohnChaouqi Misbah
Jul 16, 2008·Chemical Reviews·Christian AmatoreFrédéric Lemaître
Nov 21, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian AmatoreIrina Svir
Jun 10, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian AmatoreIrina Svir

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Citations

Apr 20, 2011·Analytical Chemistry·Yuqing LinAndrew G Ewing
Jul 5, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Raphaël Trouillon, Andrew G Ewing
Sep 24, 2016·Quarterly Reviews of Biophysics·Lin RenAndrew G Ewing
Oct 19, 2016·Accounts of Chemical Research·Xianchan LiAndrew G Ewing
Oct 7, 2016·Faraday Discussions·Jelena LovrićAndrew G Ewing
Jun 24, 2014·Scientific Reports·Takashi NakanoKeshav M Dani
Feb 2, 2019·Annual Review of Analytical Chemistry·Mimi ShinB Jill Venton
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chaoyi GuAndrew G Ewing
Mar 11, 2020·Annual Review of Analytical Chemistry·Jacqueline D KeighronAnn-Sofie Cans
Nov 10, 2012·Scientific Reports·Lisa SimonssonAnn-Sofie Cans
Sep 28, 2017·Pflügers Archiv : European journal of physiology·Hoda Fathali, Ann-Sofie Cans
Dec 4, 2012·Scientific Reports·Lisa J MellanderAndrew G Ewing
Mar 8, 2017·Proceedings. Mathematical, Physical, and Engineering Sciences·Alexander OleinickChristian Amatore
Nov 12, 2020·Chembiochem : a European Journal of Chemical Biology·Ying Wang, Andrew Ewing
Jun 9, 2018·Journal of the American Chemical Society·Mei ShenRan Chen
Apr 3, 2013·Analytical Chemistry·Raphaël Trouillon, Andrew G Ewing
Mar 27, 2015·Journal of the American Chemical Society·Johan DunevallAndrew G Ewing
Jan 10, 2014·ACS Chemical Biology·Raphaël Trouillon, Andrew G Ewing
Jan 24, 2015·Journal of the American Chemical Society·Byung-Kwon KimAllen J Bard
Jul 1, 2020·Journal of the American Chemical Society·Xiulan He, Andrew G Ewing

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