Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae.

Microbial Cell Factories
Guoqiang XuJian Chen

Abstract

Fumaric acid is a commercially important component of foodstuffs, pharmaceuticals and industrial materials, yet the current methods of production are unsustainable and ecologically destructive. In this study, the fumarate biosynthetic pathway involving reductive reactions of the tricarboxylic acid cycle was exogenously introduced in S. cerevisiae by a series of simple genetic modifications. First, the Rhizopus oryzae genes for malate dehydrogenase (RoMDH) and fumarase (RoFUM1) were heterologously expressed. Then, expression of the endogenous pyruvate carboxylase (PYC2) was up-regulated. The resultant yeast strain, FMME-001 ↑PYC2 + ↑RoMDH, was capable of producing significantly higher yields of fumarate in the glucose medium (3.18 ± 0.15 g liter-1) than the control strain FMME-001 empty vector. The results presented here provide a novel strategy for fumarate biosynthesis, which represents an important advancement in producing high yields of fumarate in a sustainable and ecologically-friendly manner.

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Citations

Feb 25, 2014·Applied Microbiology and Biotechnology·Xiaoxia YuLiming Liu
Jan 29, 2014·Applied Biochemistry and Biotechnology·Ratul Kumar Das, Satinder Kaur Brar
Jun 28, 2014·Applied Microbiology and Biotechnology·Anders G SandströmMarie F Gorwa-Grauslund
Aug 5, 2015·Metabolic Engineering·Xiulai ChenLiming Liu
Feb 25, 2015·Metabolic Engineering·Xiulai ChenLiming Liu
May 28, 2013·Metabolic Engineering·Xiulai ChenJian Chen
Sep 4, 2012·Biotechnology Advances·Qing XuJianping Wen
May 6, 2015·Chemical Society Reviews·Xinxiao SunQipeng Yuan
Apr 24, 2015·Applied Microbiology and Biotechnology·Ting ZhangYajun Yan
Apr 12, 2015·FEMS Microbiology Letters·Ting ZhangFang Wang
Jan 31, 2013·Bioresource Technology·Chunbo GuLi Deng
Dec 3, 2014·Bioresource Technology·Ning LiXueming Zhao
Apr 12, 2015·Bioresource Technology·Liang WeiJianping Wen
Dec 17, 2014·Journal of Industrial Microbiology & Biotechnology·Chandresh ThakkerGeorge N Bennett
Feb 26, 2013·Biotechnology and Bioengineering·Chan Woo SongSang Yup Lee
Sep 25, 2016·Applied and Environmental Microbiology·Dongni YanJian Chen
Nov 24, 2019·Bioprocess and Biosystems Engineering·Hasan Bugra Coban
Feb 24, 2017·Applied Microbiology and Biotechnology·Xiulai ChenLiming Liu
May 22, 2017·Applied Microbiology and Biotechnology·Jens NielsenIvan Mijakovic
Aug 31, 2017·Nature Chemical Biology·Sarah K Hammer, José L Avalos
Mar 28, 2020·Applied Microbiology and Biotechnology·Xi ZhangYu Deng
Jun 28, 2020·FEMS Yeast Research·Lisset DuranJosé L Avalos
Jun 11, 2019·Biotechnology and Bioengineering·Xiulai ChenLiming Liu
Mar 25, 2021·Journal of Bioscience and Bioengineering·Mona ChaaliMichèle Heitz

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Datasets Mentioned

BETA
HM130701
HM130702.1

Methods Mentioned

BETA
PCR

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