Reconstruction of ocular surface with heterologous limbal epithelium and amniotic membrane in a rabbit model

Cornea
M AvilaC Peña

Abstract

To report in vivo reconstruction of the ocular surface using amniotic membrane and heterologous transplants of epithelial limbal cells in rabbits with chemical burns. After severe damage to the ocular surface with n-heptanol and keratectomy, 15 rabbits developed total limbal deficiency with conjunctival epithelialization, vascularization, and chronic inflammation. One month later, a complete keratectomy was performed in all eyes: 12 received additional transplantation of human amniotic membrane and heterologous limbal epithelial cells in a double amniotic membrane layer, 2 received amniotic membrane only, and 1 control eye received no procedure. After 1 month of follow-up, corneas in eight of the operated eyes presented minimal vascularization, without signs of rejection. Corneal surface reconstruction was demonstrated with the growth of new corneal-like epithelial phenotype and integration of amniotic membrane to the basal corneal surface. A superficial amniotic membrane (with the amnion side up as a dressing) peeled off after 7 to 10 days. The epithelialization with heterologous limbal epithelial cells was evident underneath. The other four operated eyes were followed for 6 months; the ocular surface was also stable with a co...Continue Reading

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Citations

Nov 13, 2008·Cytotechnology·Marco EvangelistaOrnella Parolini
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Jan 16, 2020·International Journal of Molecular Sciences·Joan OlivaYutaka Niihara
Nov 12, 2020·Biomaterials Science·Zoe HancoxFarshid Sefat

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