PMID: 9418272Jan 7, 1998Paper

Reconstruction of optical pathlength distributions from images obtained by a wide-field differential interference contrast microscope

Journal of Microscopy
E B van MunsterJ A Aten

Abstract

An image processing algorithm is presented to reconstruct optical pathlength distributions from images of nonabsorbing weak phase objects, obtained by a differential interference contrast (DIC) microscope, equipped with a charge-coupled device camera. The method is demonstrated on DIC images of transparent latex spheres and unstained bovine spermatozoa. The images were obtained with a wide-field DIC microscope, using monochromatic light. After image acquisition, the measured intensities were converted to pathlength differences. Filtering in the Fourier domain was applied to correct for the typical shadow-cast effect of DIC images. The filter was constructed using the lateral shift introduced in the microscope, and parameters describing the spectral distribution of the signal-to-noise ratio. By varying these parameters and looking at the resulting images, an appropriate setting for the filter parameters was found. In the reconstructed image each grey value represents the optical pathlength at that particular location, enabling quantitative analysis of object parameters using standard image processing techniques. The advantage of using interferometric techniques is that measurements can be done on transparent objects, without sta...Continue Reading

Citations

Jun 18, 2002·Journal of Microscopy·E D Barone-NugentK A Nugent
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·Z KamJ W Sedat
Apr 27, 2013·BMC Bioinformatics·Boguslaw ObaraVicente Grau
May 10, 2008·Journal of Biomedical Optics·Sharon V KingChrysanthe Preza
Apr 16, 2013·Cellular and Molecular Bioengineering·Sandra M BakerOwen J T McCarty
Jul 21, 2004·Optics Letters·Christopher G RylanderAshley J Welch
Jul 1, 2014·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Sigal TrattnerNir Sochen
May 3, 2008·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·David H WojtasR P Millane
May 5, 2009·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Sigal TrattnerNir Sochen
Apr 19, 2013·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Michael Shribak
Mar 9, 2000·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·C Preza
Sep 4, 1999·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·C PrezaJ A Conchello
May 22, 2010·Applied Optics·Shalin B Mehta, Colin J R Sheppard
Jul 9, 2005·Microscopy Research and Technique·Bettina HeiseErich Peter Klement
Mar 27, 2007·Journal of Microscopy·Zvi KamJohn W Sedat
Nov 1, 2001·IEEE Transactions on Bio-medical Engineering·M J KyanC J Cogswell
Sep 30, 2005·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Feng NingPaolo Emilio Barbano
Feb 23, 2011·Biomedical Optics Express·Lisa MiccioPietro Ferraro
May 18, 2016·Medical Image Analysis·Wenchao Jiang, Zhaozheng Yin
Mar 8, 2008·Applied Optics·C C Montarou, T K Gaylord
Apr 21, 2021·Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)·Lorenzo MassimiAlessandro Olivo

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