Reconstruction of SEA-B7.1 double signals on human hepatocellular carcinoma cells and analysis of its immunological effect

Biochemical and Biophysical Research Communications
Z LiY Zheng

Abstract

Since transfection of established tumors with immunostimulatory genes, such as superantigens (SAg), a family of bacterial and viral proteins with strong immunostimulatory properties, can elicit antitumor immunity, direct transfection of tumors with genes of staphylococcal enterotoxin A (SEA) could probably set up a new way of immunological pathway. In our study, human hepatocellular carcinoma (HCC) cell lines stably transduced with SEA and B7.1/SEA fused genes, HHCCSEA and HHCCBS, were obtained by using the method of retroviral mediated gene transduction. The results showed that human HCC cells could express SEA gene. Although a tiny quantity of expression was detected, a robust immune response was promoted. The cytotoxicity of CTL on HHCCBS was the same as that on HHCCSEA. But the K(m) value of the reaction of the former was lower than that of HHCCSEA. Furthermore, the activity assay of T cells by ELISPOT demonstrated that HHCCBS could elicit more CTL activity than HHCCSEA and HHCCB7.1. It suggested that the affinity of T cells to HHCCBS was higher and the maxim velocity of reaction could be attained at an early stage of the reaction. Transduced HCC cells were also analyzed for HLA expression, and it was found that a majority ...Continue Reading

References

Jan 1, 1992·Annual Review of Immunology·J L Urban, H Schreiber
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M DohlstenT Kalland
May 11, 1990·Science·P Marrack, J Kappler
Jun 1, 1990·Science·P Marrack, J Kappler
Jan 1, 1988·Journal of Bacteriology·M J Betley, J J Mekalanos
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V A BoussiotisL M Nadler
Jul 1, 1995·Clinical Microbiology Reviews·M Kotb
Sep 1, 1995·Cancer Immunology, Immunotherapy : CII·M DohlstenT Kalland
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·M DohlstenT Kalland
Oct 1, 1993·Current Opinion in Immunology·D M Pardoll
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M DohlstenP Lind
Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·G HedlundT Kalland
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·R E TiedemannJ D Fraser
Mar 20, 1996·AIDS Research and Human Retroviruses·O Jarrett
Sep 18, 1997·The Journal of Experimental Medicine·A LalvaniA J McMichael
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·A LalvaniA V Hill
Mar 17, 1999·International Journal of Cancer. Journal International Du Cancer·A RosendahlM Dohlsten

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Citations

Oct 17, 2003·World Journal of Gastroenterology : WJG·Ning-Ling GeZhao-You Tang
Dec 27, 2003·World Journal of Gastroenterology : WJG·Shao-Ying LuYu-Song Li

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