Reconstruction of the centrosome cycle from cryoelectron micrographs

Journal of Structural Biology
D ChrétienE Karsenti

Abstract

The absence of detailed in vitro studies leaves the molecular events involved in the centrosome cycle poorly characterized. Most earlier studies have employed electron microscopy of thin or thick sections of cells. Here we have analyzed the structure of centrosomes isolated from nonsynchronized human lymphoblastic KE37 cells using cryoelectron microscopy of vitrified specimens. The centrosomes were classified into five categories depending on the number of centrioles (one or two), the respective orientation of the two centrioles in a pair (orthogonal or disoriented), and the presence or absence of appendages at the distal extremity of the centrioles (referred to as mature and immature, respectively). A detailed analysis of the centriole dimensions in these categories allowed us to reconstruct the centrosome cycle in KE37 cells. Our results suggest that centriole assembly is completed only when the mother centriole of an immature orthogonal pair separates from its daughter in preparation to centrosome duplication. Our study shows that an in vitro approach based on cryoelectron microscopy of vitrified specimens can be used to obtain detailed structural information on the centrosome cycle.

References

Mar 1, 1992·Journal of Structural Biology·M PaintrandM Bornens
Jan 1, 1990·Cell Motility and the Cytoskeleton·G Albrecht-Buehler
Jan 1, 1987·International Review of Cytology·I A Vorobjev, E S Nadezhdina
Jan 1, 1987·Cell Motility and the Cytoskeleton·M BornensE Karsenti
May 1, 1973·The Journal of Cell Biology·J B Rattner, S G Phillips
Nov 1, 1973·The Journal of Cell Biology·L G TilneyD H Snyder
Feb 1, 1968·The Journal of Cell Biology·E RobbinsA Micali
Jun 1, 1982·The Journal of Cell Biology·I A Vorobjev, Chentsov YuS
Dec 1, 1981·The Journal of Cell Biology·R Kuriyama, G G Borisy
Aug 1, 1995·The Journal of Cell Biology·B M Lange, K Gull
Jan 1, 1994·Annual Review of Biochemistry·D R KelloggB M Alberts
Jan 1, 1996·Journal of Structural Biology·J C FungD A Agard
Dec 1, 1995·Current Biology : CB·S D FullerE Karsenti
Jan 1, 1996·Cell Motility and the Cytoskeleton·G Sluder, C L Rieder
Jan 1, 1997·Biophysical Journal·R GrimmW Baumeister
Apr 1, 1993·Trends in Cell Biology·A Kalt, M Schliwa
Sep 1, 1996·Trends in Cell Biology·B M Lange, K Gull

❮ Previous
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Citations

Mar 12, 2009·Molecules and Cells·Jadranka Loncarek, Alexey Khodjakov
Feb 13, 2001·Current Opinion in Cell Biology·S K Dutcher
Jul 3, 2004·Nature Cell Biology·Marie DelattrePierre Gönczy
Nov 8, 2012·Nature Cell Biology·Jens Lüders
Jan 30, 2010·The Journal of Biological Chemistry·Felix WeisCyrille Garnier
Aug 17, 2004·DNA and Cell Biology·Qiang WangMark I Greene
Sep 24, 2010·Molecular Biology of the Cell·Nina KorzeniewskiStefan Duensing
Mar 5, 2014·The Journal of Cell Biology·Debora KellerPierre Gönczy
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michel Bornens, Pierre Gönczy
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John V Kilmartin
Mar 7, 2007·The Journal of Cell Biology·Katherine S BrownRebecca Heald
Mar 21, 2007·FEBS Letters·Rustem Uzbekov, Claude Prigent
Apr 8, 2009·The Journal of Cell Biology·Juliette AzimzadehMichel Bornens
Dec 14, 1999·The American Journal of Pathology·W L Lingle, J L Salisbury
Jul 16, 2008·Trends in Cell Biology·Petr Strnad, Pierre Gönczy
Mar 27, 2007·Trends in Cell Biology·Erich A Nigg
Oct 8, 2008·Microscopy Research and Technique·Rana IbrahimSergio Marco
Oct 7, 2011·Cytoskeleton·James E SillibourneMichel Bornens
Mar 27, 2010·The EMBO Journal·Paul GuichardAnne-Marie Tassin
Jan 3, 2006·Current Opinion in Cell Biology·Philipp J KellerErnst Hk Stelzer
Aug 9, 2003·Current Biology : CB·Alexander DammermannKaren Oegema
Dec 14, 2004·Current Biology : CB·Isabelle ArnalDenis Chrétien
Jul 13, 2001·Current Biology : CB·W F Marshall
Sep 28, 2010·Current Biology : CB·Juliette Azimzadeh, Wallace F Marshall
Mar 17, 2010·Developmental Cell·Veena SinglaJeremy F Reiter
Sep 6, 2005·Developmental Cell·Sebastian Leidel, Pierre Gönczy
Aug 8, 2007·Developmental Cell·Julia Kleylein-SohnErich A Nigg
Oct 6, 2010·Cell Division·James E Sillibourne, Michel Bornens
Mar 13, 2014·Seminars in Cell & Developmental Biology·Jens Januschke, Inke Näthke
Feb 5, 2014·Biochemical and Biophysical Research Communications·Miseon LeeKunsoo Rhee
May 25, 2016·Developmental Cell·Ashwani SharmaMichel O Steinmetz
Jun 9, 2016·Scientific Reports·Heinrich C R KleinUlrich S Schwarz
Jun 15, 2016·The Journal of Cell Biology·Andrew MuroyamaTerry Lechler
Jul 15, 2009·Seminars in Cell & Developmental Biology·Sigrid Hoyer-Fender
Sep 13, 2016·Nature Cell Biology·Audrey GuesdonDenis Chrétien

❮ Previous
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