Reconstruction technique for off-axis electron holography using coarse fringes

Ultramicroscopy
Takeshi FujitaDavid J Smith

Abstract

A reconstruction technique for off-axis electron holography not requiring Fourier transformation is presented. Background intensity and amplitude modulation recorded in a hologram are normalized using an envelope function, and a cosine-function image corresponding to interference fringes is retrieved from the hologram. A reconstructed phase image is then calculated from the retrieved cosine image. After phase unwrapping, the phases due to carrier frequency and Fresnel diffraction from the biprism are removed using a reference hologram, and the corrected phase image is obtained. One advantage of this method is that the spatial resolution does not rely on the interference fringe spacing. Another advantage is that the phase image has no artifacts due to windowing of the sideband, which occurs in the usual Fourier-transformation method. Details of the calculation process and demonstrations of the method using a latex sphere particle and self-assembled Co nanoparticles are described.

References

May 3, 2000·Journal of Electron Microscopy·K YamamotoT Hirayama
Jan 21, 2003·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Michael Lehmann, Hannes Lichte
May 12, 2005·Ultramicroscopy·D R G Mitchell, B Schaffer

❮ Previous
Next ❯

Citations

May 9, 2014·Micron : the International Research and Review Journal for Microscopy·T DenneulinJ L Rouviere
May 22, 2009·Journal of Electron Microscopy·Takeshi Fujita, Mingwei Chen
Oct 1, 2017·Scientific Reports·Nikolay CherkashinMartin J Hÿtch

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Michael Lehmann, Hannes Lichte
Journal of Electron Microscopy
Kazuo YamamotoT Hirayama
© 2021 Meta ULC. All rights reserved