Recording intracellular cAMP levels with EPAC-based FRET sensors by fluorescence lifetime imaging

Methods in Molecular Biology
Marcel RaspeKees Jalink

Abstract

Eukaryotic cells use second messengers such as Ca(2+), IP3, cGMP, and cAMP to transduce extracellular signals like hormones, via membrane receptors to downstream cellular effectors. FRET-based sensors are ideal to visualize and measure these rapid changes of second messenger concentrations in time and place. Here, we describe the use of EPAC-based FRET sensors to measure cAMP with spatiotemporal resolution in cells by fluorescence lifetime imaging (FLIM).

Citations

May 3, 2019·Communications Biology·Gail SingerCornelis J Weijer
Jan 12, 2020·Communications Biology·Gail SingerCornelis J Weijer

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