Recording large extracellular spikes in microchannels along many axonal sites from individual neurons

PloS One
Marta K LewandowskaA Hierlemann

Abstract

The numerous connections between neuronal cell bodies, made by their dendrites and axons, are vital for information processing in the brain. While dendrites and synapses have been extensively studied, axons have remained elusive to a large extent. We present a novel platform to study axonal physiology and information processing based on combining an 11,011-electrode high-density complementary metal-oxide semiconductor microelectrode array with a poly(dimethylsiloxane) channel device, which isolates axons from somas and, importantly, significantly amplifies recorded axonal signals. The combination of the microelectrode array with recording and stimulation capability with the microfluidic isolation channels permitted us to study axonal signal behavior at great detail. The device, featuring two culture chambers with over 30 channels spanning in between, enabled long-term recording of single spikes from isolated axons with signal amplitudes of 100 μV up to 2 mV. Propagating signals along axons could be recorded with 10 to 50 electrodes per channel. We (i) describe the performance and capabilities of our device for axonal electrophysiology, and (ii) present novel data on axonal signals facilitated by the device. Spontaneous action p...Continue Reading

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Citations

Mar 26, 2016·Frontiers in Neuroscience·Marta K LewandowskaAndreas R Hierlemann
Nov 11, 2017·Frontiers in Bioengineering and Biotechnology·Shailesh AppukuttanRohit Manchanda
Jan 14, 2019·Journal of Tissue Engineering and Regenerative Medicine·Dale S GeorgeStephen Lambert
Oct 29, 2019·Journal of Neural Engineering·Liang Guo
Feb 19, 2020·Journal of Biophotonics·Ida S OpstadClemens F Kaminski
Oct 26, 2018·Frontiers in Physiology·Marta K LewandowskaAnna Rostedt Punga
Jan 19, 2020·Journal of Computational Neuroscience·Harry TranValérie Louis-Dorr
May 24, 2019·Frontiers in Cellular Neuroscience·Vishalini EmmeneggerAndreas Hierlemann
Nov 1, 2017·Advanced Biosystems·Günther ZeckRoland Thewes
Jun 27, 2020·Cells·Pallavi GuptaTuhin Subhra Santra
Sep 30, 2020·Mathematical Biosciences and Engineering : MBE·Zuo Zhi LiuQuan Yuan

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Methods Mentioned

BETA
chip
confocal microscopy
chips

Software Mentioned

Fiji
NIS elements
MosaicJ

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