Recovering cellular biomass from fluids using chemical flocculation

Environmental Microbiology Reports
Paul A KenwardSean A Crowe

Abstract

We developed an efficient, scalable and inexpensive method for recovering cellular biomass from complex fluid matrices that cannot be processed using conventional filtration methods. The method uses chemical flocculation with iron oxyhydroxides, is capable of recovering greater than 90% of cellular biomass from fluids with more than 103 cells ml-1 , and was validated using both mock communities and field samples. High quality DNA can be readily extracted from iron flocs using standard soil extraction kits. We applied chemical flocculation to fracing fluids from British Columbia, Canada and recovered a diversity of microbial taxa including abundant members of the Epsilon- and Deltaproteobacteria previously recovered from shale gas operations in the United States. Application of chemical flocculation presents new opportunities for scalable time-series monitoring and experimentation on complex fluid matrices including microbial community profiling and shotgun metagenomics over gas production well completion cycles.

References

Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Apr 13, 2004·Nature Biotechnology·John F HeidelbergClaire M Fraser
May 24, 2008·Science·Paul G FalkowskiEdward F Delong
Dec 31, 2008·Applied and Environmental Microbiology·Nina SanapareddySandra M Clinton
Jan 22, 2010·The ISME Journal·Anna EngelbrektsonPhilip Hugenholtz
Jul 17, 2010·Advances in Colloid and Interface Science·Anu MatilainenMika Sillanpää
Feb 1, 2011·BMC Bioinformatics·Christopher QuincePeter J Turnbaugh
Mar 29, 2011·Current Opinion in Biotechnology·Marcus TauppSteven J Hallam
May 17, 2011·Environmental Microbiology Reports·Seth G JohnMatthew B Sullivan
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Aug 23, 2011·Applied Microbiology and Biotechnology·Lisa M GiegJulia M Foght
Jul 24, 2013·FEMS Microbiology Ecology·Arvind Murali MohanKelvin B Gregory
Oct 4, 2013·Environmental Science & Technology·Arvind Murali MohanKelvin B Gregory
Nov 10, 2013·Genome Biology·Bonnie L HurwitzMatthew B Sullivan
Dec 10, 2013·Applied and Environmental Microbiology·Dennis Enning, Julia Garrelfs
Jun 18, 2015·Frontiers in Microbiology·Cory C PadillaFrank J Stewart
Aug 11, 2016·FEMS Microbiology Ecology·Paula J MouserKelly C Wrighton
Sep 7, 2016·Nature Microbiology·Rebecca A DalyKelly C Wrighton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Genome Biology
Emmanuel F MongodinKaren E Nelson
Monographs in Human Genetics
B Tischler, R B Lowry
Canadian Medical Association Journal
J H Macdermot
Canadian Medical Association Journal
Canadian Medical Association Journal
© 2022 Meta ULC. All rights reserved