Recovery and conversion of kinins in exsanguinated rat preparations.

Naunyn-Schmiedeberg's Archives of Pharmacology
J L PradoA C Paiva

Abstract

A rat preparation in which the perfusion route bypassed the lungs, which were substituted by an artificial oxygenator, was exsanguinated and perfused with oxygenated 4% dextran (M.W. 70,000) in Tyrode's fluid; a peristaltic pump propelled the perfusing fluid at 20-25 ml/min through the aortic arch and the perfusion medium returned to the right ventricle. Known amounts of bradykinin (BK), kallidin (lysul-bradykinin, LBK) and methionyllysylbradykinin (MLBK) were administered as single injections and samples of the perfusion fluid removed between 1.5 to 6 min following injection. Average total kinin activity remaining in the circulating fluid was calculated from assays on the isolated guinea pig ileum against respective kinin injected and found to be after 3 and 6 min respectively: 20 and 5% for BK, 54 and 21% for LBK, 60 and 30% for MLBK. When the lungs were introduced in the perfusion circuit BK recoveries decreased to 0.4% at 4 min and 0% at 6 min. In 2 experiments 1 mg MLBK and, in another two, 1 mg of LBK were recirculated for 3 to 3.5 min in the rat preparation with lung bypass; enzymic reactions were interrupted in the perfusates after removal by lowering the pH to 4.7 and placing them in a boiling water bath for 5 min. Fol...Continue Reading

References

Feb 26, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·D R BorgesJ A Guimarães
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Citations

Oct 12, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·D R BorgesA C Camargo
Jan 1, 1981·The International Journal of Biochemistry·J O FreitasJ A Guimarães
May 29, 2002·Cardiovascular Research·Ervin G Erdös
Jul 27, 2005·Peptides·Maria KouyoumdjianDurval Rosa Borges
Aug 4, 2015·Biochemical Pharmacology·Diego M AssisMaria A Juliano
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Maria KouyoumdjianDurval R Borges

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