PMID: 9525616Apr 3, 1998Paper

Recovery of a fully viable chimeric human parainfluenza virus (PIV) type 3 in which the hemagglutinin-neuraminidase and fusion glycoproteins have been replaced by those of PIV type 1

Journal of Virology
T TaoBrian R Murphy

Abstract

The recent recovery of human parainfluenza virus type 3 (PIV3) from cDNA, together with the availability of a promising, highly characterized live attenuated PIV3 vaccine virus, suggested a novel strategy for the rapid development of comparable recombinant vaccine viruses for human PIV1 and PIV2. The strategy, illustrated here for PIV1, is to create chimeric viruses in which the two protective antigens, the hemagglutinin-neuraminidase (HN) and fusion (F) envelope glycoproteins, of an attenuated PIV3 variant are replaced by those of PIV1 or PIV2. As a first step, this has been achieved by using recombinant wild-type (wt) PIV3 as the recipient for PIV1 HN and F, engineered so that each PIV1 open reading frame is flanked by the existing PIV3 nontranslated regions and transcription signals. This yielded a viable chimeric recombinant virus, designated rPIV3-1, that encodes the PIV1 HN and F glycoproteins in the background of the wt PIV3 internal proteins. There were three noteworthy findings. First, in contrast to recently reported glycoprotein replacement chimeras of vesicular somatitis virus or measles virus, the PIV3-1 chimera replicates in LLC-MK2 cells and in the respiratory tract of hamsters as efficiently as its PIV1 and PIV3...Continue Reading

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Citations

Aug 4, 2015·Biological & Pharmaceutical Bulletin·Tadanobu TakahashiTakashi Suzuki
Oct 21, 1999·Microbiology and Immunology·Y Nagai, A Kato
Feb 28, 2002·Journal of Virology·Stéphane BiacchesiMichel Brémont
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Jul 2, 2011·Biological & Pharmaceutical Bulletin·Keijo FukushimaTakashi Suzuki
Nov 11, 2014·Expert Review of Vaccines·Mark A Mogler, Kurt I Kamrud
Dec 23, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Anna P Durbin, Ruth A Karron
Aug 24, 2011·Expert Review of Respiratory Medicine·Alexander C SchmidtPeter L Collins
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Jun 8, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Elliott Russell, Michael G Ison
Dec 5, 2017·Biological & Pharmaceutical Bulletin·Takashi Suzuki

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