Recovery of a neurovirulent human coronavirus OC43 from an infectious cDNA clone

Journal of Virology
Julien R St-JeanP J Talbot

Abstract

This study describes the assembly of a full-length cDNA clone of human coronavirus (HCoV)-OC43 in a bacterial artificial chromosome (BAC). The BAC containing the full-length infectious cDNA (pBAC-OC43(FL)) was assembled using a two-part strategy. The first step consisted in the introduction of each end of the viral genome into the BAC with accessory sequences allowing proper transcription. The second step consisted in the insertion of the whole HCoV-OC43 cDNA genome into the BAC. To produce recombinant viral particles, pBAC-OC43(FL) was transfected into BHK-21 cells. Recombinant virus displayed the same phenotypic properties as the wild-type virus, including infectious virus titers produced in cell culture and neurovirulence in mice.

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Citations

Apr 13, 2012·Journal of Biomedicine & Biotechnology·B Karsten Tischer, Benedikt B Kaufer
Jun 17, 2014·Virus Research·Fernando AlmazánLuis Enjuanes
Jun 27, 2014·Viruses·Christopher C Stobart, Martin L Moore
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Jul 1, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jayalakshmi VallamkonduRamesh Kandimalla
Nov 25, 2021·Journal of Virology·Makoto UjikeWataru Kamitani

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