Recovery of absorbance spectra of micrometer-sized biological and inanimate particles

The Analyst
Rozalia LukacsA Kohler

Abstract

In this paper, we first provide an overview of the Mie type scattering at absorbing materials and existing correction methods, followed by a new method to obtain the pure absorbance spectra of biological systems with spherical symmetry. This method is a further development of the recently described iterative algorithm of van Dijk et al. The method is tested on FTIR synchrotron spectra of polymethyl methacrylate (PMMA) microspheres and pollen grains with approximately spherical shape. The imaginary part of the refractive index was successfully recovered for both systems. Good agreement was obtained between the pure absorbance spectra obtained by this method and the measured spectra.

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Citations

Nov 26, 2015·Analytical Chemistry·Boris ZimmermannAchim Kohler
Apr 2, 2016·Faraday Discussions·Tatiana KonevskikhAchim Kohler
Sep 9, 2016·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·R BlümelA Kohler
Aug 10, 2017·Journal of Biophotonics·Tatiana KonevskikhAchim Kohler
Jan 17, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alistair W R SeddonBoris Zimmermann
Nov 22, 2018·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·R BlümelA Kohler
May 1, 2020·Analytical and Bioanalytical Chemistry·Sabrina DiehnJanina Kneipp
Dec 28, 2017·Analytical Chemistry·Tomasz P Wrobel, Rohit Bhargava

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