Recovery of cellular functions following oxidant injury

The American Journal of Physiology
G NowakR G Schnellmann

Abstract

This study investigated the recovery of renal proximal tubule cellular (RPTC) functions following oxidant-induced sublethal injury. tert-Butylhydroperoxide (TBHP) treatment resulted in 24% cell death and loss 4 h following the exposure. The remaining sublethally injured RPTC proliferated, and monolayer DNA content returned to control values on day 4 following TBHP exposure. Basal oxygen consumption (Qo2) and ATP content in sublethally injured RPTC were decreased 64 and 63%, respectively, at 4 h and returned to control values on day 6. Net lactate consumption decreased 71% at 4 h and returned to control values on day 4. In contrast, net glutamine consumption increased 2.7-fold at 4 h and returned to control values on day 6. Ouabain-sensitive Qo2, Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase) activity, and Na(+)-coupled glucose transport were inhibited 77, 88, and 83%, respectively, at 4 h and recovered to control values on day 6. These data show that 1) mitochondrial function, Na(+)-K(+)-ATPase activity, active Na+ transport, and Na(+)-coupled glucose transport are decreased in sublethally injured RPTC following oxidant exposure and are repaired over time; 2) monolayer regeneration precedes the recovery of mitochondrial...Continue Reading

References

Jan 1, 1992·Free Radical Biology & Medicine·C H KennedyW A Pryor
Jan 1, 1992·Kidney International·F G Toback
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C N OliverR A Floyd
Jan 1, 1991·Toxicology and Applied Pharmacology·C E GrovesR G Schnellmann
Jan 1, 1990·Archives of Toxicology·J Hannemann, K Baumann
Sep 1, 1990·The Journal of Surgical Research·D B Hinshaw, J M Burger
Dec 1, 1988·The Journal of Membrane Biology·B A MolitorisJ Kim
Apr 1, 1986·Physiological Reviews·G Wirthensohn, W G Guder
Jul 1, 1988·The American Journal of Physiology·R G Schnellmann
Sep 1, 1987·The Journal of Clinical Investigation·B A Molitoris, R Kinne
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Apr 1, 1995·The American Journal of Physiology·G Nowak, R G Schnellmann
Mar 1, 1995·Kidney International·C Wang, A K Salahudeen
Jul 1, 1994·The Korean Journal of Internal Medicine·S S ChoiY K Kim
May 15, 1996·Journal of Biotechnology·M SchneiderU von Stockar
Sep 1, 1996·Kidney International·K A NathD Warden
Dec 1, 1996·The American Journal of Physiology·G Nowak, R G Schnellmann
Jul 1, 1997·Toxicology and Applied Pharmacology·G Nowak, R G Schnellmann

❮ Previous
Next ❯

Citations

May 18, 2006·American Journal of Physiology. Renal Physiology·Grazyna NowakDiana Bakajsova
Dec 7, 2007·The Journal of Pharmacology and Experimental Therapeutics·Grazyna NowakDiana Bakajsova
Feb 13, 2008·The Journal of Pharmacology and Experimental Therapeutics·Kyle A Rasbach, Rick G Schnellmann
Jan 25, 2008·American Journal of Physiology. Renal Physiology·Lawrence H Lash
Aug 15, 2001·American Journal of Physiology. Renal Physiology·P A NonyR G Schnellmann
Dec 14, 2007·American Journal of Physiology. Renal Physiology·Zabeena P ShaikGrazyna Nowak
Feb 14, 2008·The Journal of Pharmacology and Experimental Therapeutics·Mark A HallmanRick G Schnellmann
Jun 24, 2004·American Journal of Physiology. Renal Physiology·Shougang ZhuangRick G Schnellmann
Jan 28, 2010·The Journal of Pharmacology and Experimental Therapeutics·Jason A FunkRick G Schnellmann
Oct 23, 2003·American Journal of Physiology. Renal Physiology·Grazyna NowakGinger L Clifton
Feb 27, 2003·The Journal of Pharmacology and Experimental Therapeutics·Paul A Nony, Rick G Schnellmann
Dec 5, 2003·Biochimica Et Biophysica Acta·Akira IkariKuniaki Takagi
Oct 7, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D W King, M A Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.