PMID: 8970985Dec 1, 1996Paper

Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs

Journal of Virology
G MeyersB M Kümmerer

Abstract

After cDNA cloning of the genome of bovine viral diarrhea virus (BVDV) isolate CP7, a full-length cDNA clone was constructed. RNA transcribed in vitro from this construct was shown to direct the generation of infectious BVDV upon transfection into bovine cells. To confirm the de novo generation of infectious BVDV from cloned cDNA a genetically tagged virus was constructed. In comparison with parental BVDV, the recombinant virus was slightly retarded in growth. The NS2 coding region of the CP7 genome contains a duplication of 27 nucleotides which is not present in the genome of its noncytopathogenic counterpart, NCP7. Exchange of a small fragment harboring this insertion against the corresponding part of the NCP7 sequence led to recovery of noncytopathogenic BVDV. Alteration of the construct by introduction of a fragment derived from a cytopathogenic BVDV defective interfering particle resulted in a chimeric defective interfering particle which exhibits a cytopathogenic phenotype. These findings confirm the hypothesis that the recombination-induced alterations in the genomes of cytopathogenic BVDV are responsible for the induction of cell lysis.

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Citations

Jun 20, 2001·Journal of Virology·V von MesslingR Cattaneo
Jun 26, 2009·The Journal of General Virology·Anne WegeltMartin Beer
Jan 23, 2010·The Journal of Biological Chemistry·Ioana Bintintan, Gregor Meyers
May 3, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sandra BurrackGregor Meyers
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
May 8, 1998·Journal of Viral Hepatitis·R Trowbridge, E J Gowans
Feb 26, 2005·Journal of Virology·Young-Min LeeCharles M Rice
Sep 13, 2007·The Journal of Biological Chemistry·Birke Andrea Tews, Gregor Meyers
Jan 8, 2010·The Journal of General Virology·Martina BarothSven-Erik Behrens
Nov 11, 2014·Expert Review of Vaccines·Mark A Mogler, Kurt I Kamrud
Mar 12, 2020·Microbial Genomics·George C RussellClaudia Bachofen
Dec 21, 2007·Journal of Virology·Donna M TscherneCharles M Rice
Nov 14, 2019·Veterinary Sciences·Anna MichelitschMartin Beer
Jul 1, 2008·Nature Reviews. Microbiology·Catherine L MurrayCharles M Rice

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