Recovery of dengue virus from urine samples by real-time RT-PCR

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
D Van den BosscheMarjan Van Esbroeck

Abstract

Recently, reverse transcription polymerase chain reaction (RT-PCR) for dengue virus (DENV) has been reported to test positive in urine samples for a longer time frame than in serum. We evaluated two RNA extraction procedures from urine and investigated the stability of DENV RNA in urine and serum up to 1 year at different storage temperatures. In addition, 24 urine samples collected from patients with a recent infection were tested with DENV real-time RT-PCR and compared to the RT-PCR results on serum. Five patients with an acute DENV infection were followed up for 6 months by RT-PCR on urine. The automated extraction method with the MagNA Pure LC 2.0 device had a higher yield of DENV RNA compared to the manual QIAGEN method, explained by the higher volume used in the former method. DENV RNA in both serum and urine was stable at room temperature up to 1 month and at 4 °C and -20 °C for at least 1 year. The detection rate by RT-PCR on urine was 50 % (4/8) until day 7, 100 % (6/6) between 1 and 3 weeks after symptom onset, and 25 % (2/8) thereafter. Generally, DENV RNA concentrations are higher in serum than in urine up till day 7, switching to lower concentrations in serum thereafter. Peak concentrations in urine are reached aro...Continue Reading

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Citations

Apr 30, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Birgit De SmetLieselotte Cnops
Jun 6, 2014·Epidemiology and Infection·P Sendi, S Fröhlicher
May 21, 2016·Lancet·Martin Peter GrobuschAbraham Goorhuis
Oct 21, 2016·Journal of the Pediatric Infectious Diseases Society·Lucille ArragainElodie Descloux
Dec 12, 2017·Open Forum Infectious Diseases·G Khai Lin HuangJulian Druce
Mar 23, 2019·BMJ Global Health·Laura T Mazzola, Cassandra Kelly-Cirino
Mar 19, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Dilek Yagci-CaglayikAykut Ozkul
Feb 15, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jean X Y SimJenny G-H Low

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