Recovery of functional cytotoxic T lymphocytes during lamivudine therapy by acquiring multi-specificity

Journal of Medical Virology
Yasuteru KondoTooru Shimosegawa

Abstract

To characterize cytotoxic T lymphocytes (CTLs) that appeared in circulation during lamivudine therapy, we analyzed HBV-specific CTLs using HLA-A24 tetramer and HBcAg-specific Th1 cells in patients receiving lamivudine therapy. Six patients (HLA-A24(+)) with chronic hepatitis B, six patients (HLA-A24(-)) with chronic hepatitis B, and six patients (HLA-A24(+)) with chronic hepatitis C were studied. In addition to known CTL epitopes (C117 and P756), three epitopes were confirmed as CTL epitopes (C23, S89, S226) by chromium release assay and by staining intracellular perforin. CTLs specific for P756 were most frequently found at pre-treatment. During lamivudine therapy, increase in the frequencies of HLA-tetramer(+) cells was found for C117, S89, and S226. Recovery of CTLs was observed earlier in patients with HBeAg(-)/anti-HBe(+) compared with those with HBeAg(+)/anti-HBe(-). HBcAg-specific Th1 cells did not increase significantly up to 8 weeks. T cell lines from patients with chronic hepatitis B had a lower level of proliferation (0- to 24.9-fold expansion by in vitro stimulation) and a higher ability to produce interferon-gamma (0-84% except for S89), while perforin-positive cells showed low frequencies (0-50% except for S89). I...Continue Reading

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Citations

Jul 10, 2010·Journal of Biomedicine & Biotechnology·Wan-Yu LiJun-Qi Niu
Feb 13, 2013·ISRN Gastroenterology·Yasuteru KondoTooru Shimosegawa
Dec 23, 2011·Gastroenterology Research and Practice·Yasuteru KondoTooru Shimosegawa
May 17, 2012·Journal of Gastroenterology·Yasuteru KondoTooru Shimosegawa
Nov 6, 2007·Clinics in Liver Disease·John M Vierling
Jun 19, 2013·Immunological Reviews·Christina C ChangSharon R Lewin
Apr 10, 2015·Clinical Journal of Gastroenterology·Yasuteru KondoTooru Shimosegawa
Apr 17, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Nan MengWang-Xian Tang
Jul 26, 2006·World Journal of Gastroenterology : WJG·Yasuteru KondoTooru Shimosegawa

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