Recovery of IFN-gamma levels in PBMCs from lepromatous leprosy patients through the synergistic actions of the cytokines IL-12 and IL-18

International Immunopharmacology
Rocio Ivette Lopez RoaMary Fafutis Morris

Abstract

The shift to the production of a Th1 cytokine profile during an intracellular infection has been shown to depend on antigen presenting cells-derived IL-12 and T-cell-derived IFN-gamma production. IL-18 facilitates Th1 priming in synergy with IL-12 through the stimulation of IFN-gamma production by T cells, B cells, NK cells, macrophages and DCs. A low level of IFN-gamma production in PBMC cultures from lepromatous leprosy patients (LL) has been previously reported by several groups. We evaluated the synthesis of this cytokine after exogenous addition of recombinant IL-12 and IL-18 (IL12/IL18) in order to induce recovery of the IFN-gamma levels with Mycobacterium leprae antigenic stimulation. The aim of this study was to investigate if exogenous addition of IL12/IL18 to PBMC cell cultures in the presence of M. leprae antigens could induce recovery of IFN-gamma levels. We found that IFN-gamma levels in PBMCs cultured from LL patients were reestablished after exogenous addition of exogenous IL12/IL18 and we also observed a diminished IL-18R expression. Although the molecular mechanisms of IL12/IL18 synergy have not been clearly elucidated, we assume that recombinant cytokines can activate several transcription factors that induce ...Continue Reading

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Citations

Apr 30, 2010·Clinical and Vaccine Immunology : CVI·Annemieke GelukTom H M Ottenhoff
Apr 9, 2013·Revista Da Sociedade Brasileira De Medicina Tropical·Ana Carolina Fragoso MottaNorma Tiraboschi Foss
Dec 31, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Celia Guerrero-VelázquezMary Fafutis-Morris
Oct 12, 2018·Journal of Applied Oral Science : Revista FOB·Ramón Franco-TopeteCelia Guerrero-Velázquez
Feb 22, 2011·Oral Diseases·P E Sánchez-HernándezC Guerrero-Velázquez

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