Recovery of ionizing-radiation damage after high doses of gamma ray in the hyperthermophilic archaeon Thermococcus gammatolerans.

Extremophiles : Life Under Extreme Conditions
Angels TapiasFabrice Confalonieri

Abstract

The recently discovered hyperthermophilic and radioresistant archaeon Thermococcus gammatolerans is of great interest to compare and contrast the impact of its physiology on radioresistance and its ability to repair damaged chromosomes after exposure to gamma irradiation with radioresistant bacteria. We showed that, in contrast to other organisms, cell survival was not modified by the cellular growth phase under optimal growth conditions but nutrient-limited conditions did affect the T. gammatolerans radioresistance. We determined the first kinetics of damaged DNA recovery in an archaeon after exposure to massive doses of gamma irradiation and compared the efficiency of chromosomal DNA repair according to the cellular growth phase, nutrient availability and culture conditions. Chromosomal DNA repair kinetics showed that stationary phase cells reconstitute disrupted chromosomes more rapidly than exponential phase cells. Our data also revealed that this radioresistant archaeon was proficient to reconstitute shattered chromosomes either slowly or rapidly without any loss of viability. These results suggest that rapid DNA repair is not required for the extreme radioresistance of T. gammatolerans.

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Citations

Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Athanasia PavlopoulouAlexandros G Georgakilas
Sep 28, 2016·Chemical Research in Toxicology·Ewa BarbierJean Breton
Nov 28, 2017·Annual Review of Genetics·Mar Martinez-PastorAmy K Schmid
Jul 1, 2017·The Journal of Microbiology·Kwang-Woo JungYong-Sun Bahn
Aug 2, 2017·Cancer Nanotechnology·Sha LiSandrine Lacombe
Nov 24, 2018·Extremophiles : Life Under Extreme Conditions·A GorlasP Forterre

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