Recovery of prostacyclin production by de-endothelialized rabbit aorta. Critical role of neointimal smooth muscle cells

The Journal of Clinical Investigation
A EldorB B Weksler

Abstract

Prostacyclin (PGI2) synthetic capacity was assayed at the surface of aortas at various intervals after removal of endothelium with a balloon catheter. Results were correlated with morphologic changes in the vessel wall seen by light microscopy, scanning and transmission electron microscopy. To assay PGI2 synthetic capacity, we applied an incubation chamber to the luminal surface of the aortas; after arachidonic acid stimulation we assayed the PGI2 synthesized with a bioassay and radioimmunoassay. PGI2 synthesis in de-endothelialized aortas was determined immediately after balloon-catheter injury and at intervals of 1 h and 2, 4, 15, 35, and 70 d. PGI2 synthesis was low at 1 h and increased over time with levels at 35 and 70 d reaching that of normal artery. Scanning and transmission electron microscopy of de-endothelialized areas showed persistent absence of endothelium with formation of a neointima composed of smooth muscle cells. De-endothelialized aorta was covered with adherent platelets shortly after injury, however several days later only a few platelets adhered to the denuded surface. Results indicated that (a) endothelium is responsible for nearly all PGI2 production at the luminal surface of the normal aorta, (b) de-en...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B B WekslerE A Jaffe
Nov 1, 1978·The Journal of Clinical Investigation·B B WekslerE A Jaffe
Jan 1, 1979·Thrombosis Research·E G Levin, D J Loskutoff
Jan 1, 1979·Haemostasis·J J Emeis
Jan 1, 1977·Haemostasis·F Markwardt, H P Klöcking
Sep 9, 1977·Biochemical and Biophysical Research Communications·N L BaenzigerP W Majerus
Oct 1, 1972·The Journal of Experimental Medicine·M B Stemerman, R Ross

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Citations

Mar 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·M RampartA G Herman
Apr 1, 1985·Experimental and Molecular Pathology·G R Campbell, J H Campbell
Oct 1, 1983·Prostaglandins·J M Boeynaems, N Galand
Jul 1, 1986·Prostaglandins·D Demolle, J M Boeynaems
Jan 1, 1987·Pharmacology & Therapeutics·R J MorinS K Peng
Jan 1, 1989·Pharmacology & Therapeutics·J A Angus, T M Cocks
Jan 1, 1990·Pharmacology & Therapeutics·G J Dusting, P S MacDonald
Jan 1, 1987·Progress in Lipid Research·W S Powell, C D Funk
Jan 18, 1988·Biochimica Et Biophysica Acta·G M Rodgers
Apr 1, 1987·Prostaglandins, Leukotrienes, and Medicine·E A Lennon, N L Poyser
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·J R Jauchem
Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·G E ThompsonM Peaker
Jan 1, 1983·General Pharmacology·E A Higgs, S Moncada
Mar 1, 1990·Journal of the American College of Cardiology·T F LüscherC Boulanger
Mar 1, 1990·Journal of the American College of Cardiology·M L KnudtsonH J Duff
Jan 1, 1989·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P Vallance
Aug 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·R Imai-SasakiT Nakadate
Apr 1, 1995·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·G J CooperG D Angelini
Nov 1, 1993·Journal of Pharmacological and Toxicological Methods·M GabaldónA Zúñiga
Aug 1, 1996·American Journal of Surgery·J A ManiconeV Parsonnet
Dec 1, 1986·Journal of the American College of Cardiology·L A Harker, V Fuster
Jan 1, 1997·Free Radical Biology & Medicine·L A Bharadwaj, K Prasad
Nov 1, 1992·European Journal of Vascular Surgery·E MattssonD Bergqvist
May 1, 1997·British Journal of Pharmacology·D Bishop-BaileyJ A Mitchell
Sep 15, 1983·The New England Journal of Medicine
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S R CoughlinL Levine
Apr 1, 1986·Annals of Surgery·V J HendersonD C Miller
Apr 1, 1996·Plastic and Reconstructive Surgery·R J RestifoP C Johnson
Aug 1, 1983·The Journal of Clinical Investigation·R S KentA R Whorton
Jul 1, 1985·The Journal of Clinical Investigation·J M BoeynaemsP Ketelbant
Feb 17, 1998·International Journal of Cardiology·D MehtaG D Angelini
Aug 1, 1987·Blut·H A Bull, S J Machin

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