Recruitment of Cdc20 to the kinetochore requires BubR1 but not Mad2 in Drosophila melanogaster

Molecular and Cellular Biology
Deyu LiJunyong Huang


To prevent aneuploidy, cells require a mitotic surveillance mechanism, the spindle assembly checkpoint (SAC). The SAC prevents metaphase/anaphase transition by blocking the ubiquitylation and destruction of cyclin B and securin via the Cdc20-activated anaphase-promoting complex or cyclosome (APC/C)-mediated proteolysis pathway. This checkpoint involves the kinetochore proteins Mad2, BubR1, and Cdc20. Mad2 and BubR1 are inhibitors of the APC/C, but Cdc20 is an activator. Exactly how the SAC regulates Cdc20 via unattached kinetochores remains unclear; in vertebrates, most current models suggest that kinetochore-bound Mad2 is required for initial binding to Cdc20 to form a stable complex that includes BubR1. Here, we show that the Mad2 kinetochore dimerization recruitment mechanism is conserved and that the recruitment of Cdc20 to kinetochores in Drosophila requires BubR1 but not Mad2. BubR1 and Mad2 can bind to Cdc20 independently, and the interactions are enhanced after cells are arrested at mitosis by the depletion of Cdc27 using RNA interference (RNAi) in S2 cells or by MG132 treatment in syncytial embryos. These findings offer an explanation of why BubR1 is more important than Mad2 for SAC function in flies. These findings co...Continue Reading


Feb 27, 2013·Molecular and Cellular Biology·Heather DeBruhlJoseph S Lipsick
Sep 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joo Seok HanD W Cleveland
Nov 3, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jakob Nilsson
Mar 14, 2020·PLoS Genetics·Zsolt G VenkeiYukiko M Yamashita
Jul 13, 2017·Genes & Development·Taekyung KimArshad Desai
Jan 1, 2015·Molecular & Cellular Oncology·Tiziana Lischetti, Jakob Nilsson
Nov 3, 2020·PLoS Genetics·Damien GarridoVincent Archambault


Jun 12, 1998·The Journal of Cell Biology·J C WatersEdward D Salmon
Mar 4, 2000·Nature Structural Biology·Xuelian LuoG Wagner
Feb 13, 2001·Cell·J V Shah, D W Cleveland
May 15, 2002·Molecular Biology of the Cell·Eunah Chung, Rey-Huei Chen
Jun 26, 2002·The Journal of Cell Biology·Jordan W RaffJunyong Huang
Aug 28, 2002·The Journal of Cell Biology·Marko J KallioGary J Gorbsky
Oct 22, 2002·Molecular Biology of the Cell·Katie B ShannonEdward D Salmon
Dec 11, 2002·Current Opinion in Cell Biology·Hongtao Yu
Jan 9, 2004·Current Biology : CB·Renata BastoRoger E Karess
Mar 17, 2004·Nature Structural & Molecular Biology·Xuelian LuoHongtao Yu
Jun 9, 2004·Current Biology : CB·Bonnie J HowellEdward D Salmon
Jul 23, 2004·Molecular Biology of the Cell·Suzanne VigneronThierry Lorca
Feb 8, 2005·Current Biology : CB·Anna De AntoniAndrea Musacchio
Mar 31, 2005·Cell·Kim Nasmyth
May 12, 2005·Current Biology : CB·Eulalie BuffinRoger E Karess
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anna DeAntoniAndrea Musacchio
Apr 8, 2006·Experimental Cell Research·James DavenportRakesh Goorha
Jun 7, 2006·Current Biology : CB·Péter Lénárt, Jan-Michael Peters
Aug 10, 2006·Nature Reviews. Molecular Cell Biology·Jan-Michael Peters
Apr 7, 2007·Science·Gohta GoshimaNico Stuurman
Apr 10, 2007·Nature Cell Biology·Eulalie BuffinRoger E Karess
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon

Related Concepts

Biochemical Pathway
Fzy protein, Drosophila
BUB1B gene
Cell Cycle Proteins
Chimeric Proteins, Recombinant
Cdc20 Proteins
Drosophila melanogaster Proteins

Related Feeds

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.