Recruitment of human TBP selectively activates RNA polymerase II TATA-dependent promoters.

The Journal of Biological Chemistry
B MajelloL Lania

Abstract

An increasing body of evidence suggests that eukaryotic activators stimulate polymerase II transcription by facilitating the assembly of the functional basal machinery at the promoter. Here we describe experiments that provide added support for the idea that recruitment of TATA-binding protein (TBP) is a rate-limiting step for transcription activation in mammalian cells. We found that, in human cell lines, recruitment of TBP to a promoter, as a GAL4-TBP fusion protein, can provide a substantial activation of transcription. Activation mediated by the hTBP, tethered to promoter DNA, is strictly dependent upon the presence of a functional TATA element, and it directs faithful transcription initiation. Interestingly, GAL4-hTBP activation was not observed from initiator (Inr) -dependent TATA-less promoters. These results suggest that TBP binding to DNA is not a rate-limiting step for the initial stages of TFIID recruitment to initiator-dependent TATA-less promoters. Finally, we provide evidence that synergy between GAL4-hTBP and defined transcription domains is restricted to activators, such as VP16 and Tat, which are likely to function at steps subsequent to the TFIID recruitment. These findings strengthen the idea that recruitment...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Weis, D Reinberg
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J CarcamoD Reinberg
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S T SmaleD Baltimore
Apr 7, 1989·Cell·S T Smale, D Baltimore
Mar 2, 1989·Nature·J W Lillie, M R Green
Jan 1, 1995·Annual Review of Biochemistry·L Zawel, D Reinberg
Mar 1, 1995·Trends in Biochemical Sciences·A J Koleske, R A Young
Jan 1, 1994·Annual Review of Biochemistry·K A Jones, B M Peterlin
Jan 1, 1994·Molecular and Cellular Biology·R JavaheryS T Smale
Jul 1, 1993·Genes & Development·N Hernandez
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·E MartinezR G Roeder
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·G Pengue, L Lania
Apr 12, 1996·The Journal of Biological Chemistry·P De LucaL Lania
May 1, 1996·Molecular and Cellular Biology·J BlauD Bentley
Aug 1, 1996·Trends in Genetics : TIG·L A Stargell, K Struhl
Sep 15, 1996·Genes & Development·S FarrellM Ptashne
Apr 1, 1996·Genes & Development·J KaufmannS T Smale
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg

❮ Previous
Next ❯

Citations

Mar 10, 2000·Biochemical and Biophysical Research Communications·J M KimS Kim
Jul 17, 2001·Biochemical and Biophysical Research Communications·T OhbayashiT A Tamura
Apr 25, 2013·Nature Reviews. Cancer·Megan J BywaterRoss D Hannan
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Robert J White
Apr 17, 2003·Molecular and Cellular Biology·Sandra A S JohnsonDeborah L Johnson
Apr 16, 2005·Molecular and Cellular Biology·Meghana M Kulkarni, David N Arnosti
Nov 11, 2005·Retrovirology·John Brady, Fatah Kashanchi
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·E A Felinski, P G Quinn
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J NevadoM Ptashne
Mar 16, 2011·FEBS Letters·Ann Florence B VictorianoTakashi Okamoto
Mar 28, 2008·Biochemical and Biophysical Research Communications·Inken Wierstra
Aug 29, 1998·The Journal of Biological Chemistry·H Xiao, K T Jeang
May 21, 1999·The Journal of Biological Chemistry·C J FryP J Farnham
Feb 14, 2006·Molecular Endocrinology·Alicja J CopikE Brad Thompson
Jun 22, 1999·Molecular and Cellular Biology·C Suñé, M A Garcia-Blanco
Jan 23, 1999·Biological Chemistry·A Barberis, L Gaudreau

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.