Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.

Nucleic Acids Research
Eve-Lyne MathieuAlexander Brehm

Abstract

The ATP-dependent chromatin remodeler dMi-2 can play both positive and negative roles in gene transcription. Recently, we have shown that dMi-2 is recruited to the hsp70 gene in a heat shock-dependent manner, and is required to achieve high transcript levels. Here, we use chromatin immunoprecipitation sequencing (ChIP-Seq) to identify other chromatin regions displaying increased dMi-2 binding upon heat shock and to characterize the distribution of dMi-2 over heat shock genes. We show that dMi-2 is recruited to the body of at least seven heat shock genes. Interestingly, dMi-2 binding extends several hundred base pairs beyond the polyadenylation site into the region where transcriptional termination occurs. We find that dMi-2 does not associate with the entire nucleosome-depleted hsp70 locus 87A. Rather, dMi-2 binding is restricted to transcribed regions. Our results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.

References

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Citations

Feb 26, 2016·Cell & Bioscience·Aleksey N KrasnovNadezhda E Vorobyeva
Mar 26, 2013·Methods : a Companion to Methods in Enzymology·Vladimir B TeifKarsten Rippe
Jan 8, 2013·Development·Nicola ReynoldsBrian Hendrich

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Methods Mentioned

BETA
PARylation
transgenic
immunoprecipitation
ChIP-Seq
FCS
immunoprecipitations
ChIP
RNA-seq
ribosylation

Software Mentioned

LOEWE
Ensembl
mfold
Bowtie
DESeq
Kismet
MACS

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