Rectal Swabs as an Alternative Sample Collection Method to Bulk Stool for the Real-Time PCR Detection of Giardia duodenalis

The American Journal of Tropical Medicine and Hygiene
Jacqueline R M A MaaschKristen Aiemjoy

Abstract

Though bulk stool remains the gold standard specimen type for enteropathogen diagnosis, rectal swabs may offer comparable sensitivity with greater ease of collection for select pathogens. This study sought to evaluate the validity and reproducibility of rectal swabs as a sample collection method for the molecular diagnosis of Giardia duodenalis. Paired rectal swab and bulk stool samples were collected from 86 children ages 0-4 years living in southwest Niger, with duplicate samples collected among a subset of 50 children. Infection was detected using a previously validated real-time PCR diagnostic targeting the small subunit ribosomal RNA gene. Giardia duodenalis was detected in 65.5% (55/84) of bulk stool samples and 44.0% (37/84) of swab samples. The kappa evaluating test agreement was 0.81 (95% CI: 0.54-1.00) among duplicate stool samples (N = 49) and 0.75 (95% CI: 0.47-1.00) among duplicate rectal swabs (N = 48). Diagnostic sensitivity was 93% (95% CI: 84-98) by bulk stool and 63% (95% CI: 49-75) by rectal swabs. When restricting to the lowest three quartiles of bulk stool quantitation cycle values (an indication of relatively high parasite load), sensitivity by rectal swabs increased to 78.0% (95% CI: 64-89, P < 0.0001). T...Continue Reading

References

Jan 1, 1996·The Journal of Infectious Diseases·C L ChappellH L DuPont
Feb 8, 2003·Journal of Clinical Microbiology·Stephanie P JohnstonPatricia P Wilkins
Mar 11, 2003·Transactions of the Royal Society of Tropical Medicine and Hygiene·Lorenzo SavioliAli Mohammed Shein
Oct 29, 2003·Molecular and Cellular Probes·Jaco J VerweijAnton M Polderman
Apr 28, 2009·Epidemiology and Infection·S J SnelJ Learmonth
Apr 20, 2010·Nature Reviews. Microbiology·Johan AnkarklevStaffan G Svärd
Sep 10, 2010·The Journal of Pediatrics·Bruno P ChumpitaziRobert J Shulman
May 6, 2011·BMC Microbiology·Zahra Faghiri, Giovanni Widmer
Jun 21, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Lars GustavssonMagnus Lindh
Aug 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sirisha KundrapuCurtis J Donskey
Oct 22, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Wences ArveloKim A Lindblade
Nov 6, 2013·Biotechnology Advances·Anson V KoehlerRobin B Gasser
Jun 20, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J A SidlerA F Widmer
Jul 16, 2014·PloS One·Andries E BuddingPaul H M Savelkoul
Dec 1, 2015·MMWR. Surveillance Summaries : Morbidity and Mortality Weekly Report. Surveillance Summaries·Karen PazolCenters for Disease Control and Prevention (CDC)
Mar 18, 2016·Parasite : Journal De La Société Française De Parasitologie·Martin F Heyworth
Nov 5, 2016·Current Opinion in Microbiology·Elin EinarssonStaffan G Svärd
Mar 16, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hamish McCallumJoanne Lello
Apr 1, 2017·BMC Microbiology·Christine M BassisCDC Prevention Epicenters Program
Jul 18, 2017·The Lancet. Gastroenterology & Hepatology·Stephen B FreedmanAlberta Provincial Pediatric EnTeric Infection TEam (APPETITE)
Aug 13, 2017·Journal of Infection and Public Health·Sanja GlisovicIvan Brukner
Sep 14, 2017·Revista Do Instituto De Medicina Tropical De São Paulo·Flávia Fernandes de Mendonça UchôaNádia Regina Pereira Almosny
Nov 7, 2017·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Moustafa GiborySusanne Gjeruldsen Dudman
Dec 12, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Simone M CacciòStaffan G Svärd
Apr 26, 2018·The New England Journal of Medicine·Jeremy D KeenanMORDOR Study Group
May 4, 2018·Journal of Clinical Microbiology·N JazmatiH Seifert
Apr 5, 2019·Journal of Clinical Microbiology·Jianling XieStephen B Freedman
Dec 6, 2019·Current Diabetes Reports·Chun-Heng Kuo, Hung-Yuan Li

Citations

Methods Mentioned

BETA
PCR

Clinical Trials Mentioned

NCT02048007

Related Concepts

Diagnosis
Giardia lamblia
Parasites
Rectum
Genes, rRNA
Evaluation
Finding
Detected (Finding)
Rectal Swab (Specimen)
Detection

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