Rectified cell migration on saw-like micro-elastically patterned hydrogels with asymmetric gradient ratchet teeth

PloS One
Satoru Kidoaki, Hiroyuki Sakashita

Abstract

To control cell motility is one of the essential technologies for biomedical engineering. To establish a methodology of the surface design of elastic substrate to control the long-range cell movements, here we report a sophisticated cell culture hydrogel with a micro-elastically patterned surface that allows long-range durotaxis. This hydrogel has a saw-like pattern with asymmetric gradient ratchet teeth, and rectifies random cell movements. Durotaxis only occurs at boundaries in which the gradient strength of elasticity is above a threshold level. Consequently, in gels with unit teeth patterns, durotaxis should only occur at the sides of the teeth in which the gradient strength of elasticity is above this threshold level. Therefore, such gels are expected to support the long-range biased movement of cells via a mechanism similar to the Feynman-Smoluchowski ratchet, i.e., rectified cell migration. The present study verifies this working hypothesis by using photolithographic microelasticity patterning of photocurable gelatin gels. Gels in which each teeth unit was 100-120 µm wide with a ratio of ascending:descending elasticity gradient of 1:2 and a peak elasticity of ca. 100 kPa supported the efficient rectified migration of 3T3...Continue Reading

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Citations

May 24, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Thasaneeya KubokiSatoru Kidoaki
Nov 29, 2015·Trends in Cell Biology·David CaballeroDaniel Riveline
Aug 20, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Keiichiro KushiroMadoka Takai
May 22, 2018·Biomaterials Research·Elisabeth B EvansDiane Hoffman-Kim
Dec 4, 2019·Advanced Healthcare Materials·Céline KayalJames B Phillips
Sep 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Raquel SilvaAldo R Boccaccini
May 19, 2019·Biophysics Reviews·Satoru Kidoaki
Feb 3, 2019·Biophysical Journal·Brian J DuChezKenneth M Yamada

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Methods Mentioned

BETA
irradiating
atomic force microscopy
AFM
confocal microscopy
Phase

Software Mentioned

ImageJ

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