Recurrent Duplication and Diversification of Acrosomal Fertilization Proteins in Abalone

BioRxiv : the Preprint Server for Biology
Jolie A CarlisleWillie J Swanson

Abstract

Reproductive proteins mediating fertilization commonly exhibit rapid sequence diversification driven by positive selection. This pattern has been observed among nearly all taxonomic groups, including mammals, invertebrates, and plants, and is remarkable given the essential nature of the molecular interactions mediating fertilization. Gene duplication is another important mechanism that facilitates the generation of molecular novelty. Following duplication, paralogs may parse ancestral gene function (subfunctionalization) or acquire new roles (neofunctionalization). However, the contributions of duplication followed by sequence diversification to the molecular diversity of gamete recognition genes has been understudied in many models of fertilization. The marine gastropod mollusk abalone is a classic model for fertilization. Its two acrosomal proteins (lysin and sp18) are ancient gene duplicates with unique gamete recognition functions. Through detailed genomic and bioinformatic analyses we show how duplication events followed by sequence diversification has played an ongoing role in the evolution of abalone acrosomal proteins. The common ancestor of abalone had four members of its acrosomal protein family in a tandem gene array...Continue Reading

Datasets Mentioned

BETA
MN102340-42
HM582239

Methods Mentioned

BETA
NMR
PCR
density gradient centrifugation

Software Mentioned

Crux
RAxML
tBlastn
Samtools faidx
Pal2Nal sever
Protein2Genome
PAML
PROMALS3D
SignalP
Exonerate

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