DOI: 10.1101/471243Nov 16, 2018Paper

Recurrent losses and rapid evolution of the condensin II complex in insects

BioRxiv : the Preprint Server for Biology
Thomas D KingNitin Phadnis

Abstract

Condensins play a crucial role in the organization of genetic material by compacting and disentangling chromosomes. The condensin I and condensin II complexes are widely considered to have distinct functions based on studies in a few model organisms, although the specific functions of each complex are yet to be fully understood. The condensin II complex is critical for genome organization in Drosophila, and is a key anti-pairing factor that separates homologous chromosomes in somatic cells. Intriguingly, the Cap-G2 subunit of condensin II is absent in Drosophila melanogaster, and this loss may be related to the high levels of homologous chromosome pairing in somatic cells seen in flies. Here, we find that this Cap-G2 loss predates the origin of Dipterans, and other CapG2 losses have occurred independently in multiple insect lineages. Furthermore, the Cap-H2 and Cap-D3 subunits have also been repeatedly and independently lost in several insect orders, and some taxa lack condensin II-specific subunits entirely. We used Oligopaint DNA-FISH to quantify pairing levels in ten species across seven orders, representing several different configurations of the condensin II complex. We find that all non-Dipteran insects display near-unifo...Continue Reading

Related Concepts

Capsule (Pharmacologic)
Chromosomes
Diptera
Drosophila
Drosophila melanogaster
Genome
Hydrogen Sulfide
Insecta
Fluorescent in Situ Hybridization
Condensin complexes

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