Recurrent micronucleation through cell cycle progression in the presence of microtubule inhibitors

Cell Structure and Function
Yuji NakayamaM Oshimura

Abstract

Although most cell lines undergo mitotic arrest after prolonged exposure to microtubule inhibitors, some cells subsequently exit this state and become tetraploid. Among these cells, limited numbers of rodent cells are known to undergo multinucleation to generate multiple small independent nuclei, or micronuclei by prolonged colcemid treatment. Micronuclei are thought to be formed when cells shift to a pseudo G1 phase, during which the onset of chromosomal decondensation allows individual chromosomes distributed throughout the cell to serve as sites for the reassembly of nuclear membranes. To better define this process, we used long-term live cell imaging to observe micronucleation induced in mouse A9 cells by treating with the microtubule inhibitor colcemid. Our observations confirm that nuclear envelope formation occurs when mitotic-arrested cells shift to a pseudo G1 phase and adopt a tetraploid state, accompanied by chromosome decondensation. Unexpectedly, only a small number of cells containing large micronuclei were formed. We found that tetraploid micronucleated cells proceeded through an additional cell cycle, shifting to a pseudo G1 phase and forming octoploid micronucleated cells that were smaller and more numerous com...Continue Reading

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Citations

Feb 7, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Mitsuo OshimuraToshiaki Inoue
May 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuji Nakayama, Toshiaki Inoue
Jul 7, 2016·Molecular Therapy. Methods & Clinical Development·Mikhail LiskovykhNatalay Kouprina
Oct 30, 2016·ACS Synthetic Biology·Naoki WadaMitsuo Oshimura
Oct 5, 2020·Cellular and Molecular Life Sciences : CMLS·Sergey A SinenkoAlexey N Tomilin
Feb 25, 2020·Experimental Cell Research·Teruhiko SuzukiMitsuo Oshimura

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