Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides

Methods in Molecular Biology
Gregory LinshizEhud Shapiro

Abstract

Making faultless complex objects from potentially faulty building blocks is a fundamental challenge in computer engineering, nanotechnology, and synthetic biology. We developed an error-correcting recursive construction procedure that attempts to address this challenge. Making DNA molecules from synthetic oligonucleotides using the procedure described here surpasses existing methods for de novo DNA synthesis in speed, precision, and amenability to automation. It provides for the first time a unified DNA construction platform for combining synthetic and natural DNA fragments, for constructing designer DNA libraries, and for making the faultless long synthetic DNA building blocks needed for de novo genome construction.

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

Related Papers

Systems and Synthetic Biology
Uri ShabiEhud Shapiro
Current Opinion in Biotechnology
P Holliger, G Winter
Current Opinion in Biotechnology
Claudio Mussolino, Toni Cathomen
© 2022 Meta ULC. All rights reserved