Recycling of cellulases in lignocellulosic hydrolysates using alkaline elution

Bioresource Technology
Ana Cristina RodriguesMiguel Gama

Abstract

The recovery of cellulases from lignin, lignocellulosic hydrolysates and cellulose by alkaline washes at pH 9 and 10 was examined. The effect of the pH on the structural stability of purified Cel7A was analyzed by circular dichroism. Purified Cel7A showed conformational changes at pH 9 and 10 that were reversible at pH 4.8. Temperature influenced the enzymatic hydrolysis of wheat straw and may be critical for the efficiency of cellulase recycling from wheat straw hydrolysates. Operation at moderate temperatures (37°C) resulted in a rate of saccharification 19% higher than that obtained at 50° C, improving cellulase recycling by 49%. Over 60% of the enzyme activity on the synthetic substrate 4-methylumbelliferyl-β-d-cellobioside (MUC) may be recovered by using a simple alkaline wash. This is thus a promising strategy for enzyme recycling that is simple to implement at industrial scale, economical and effective.

References

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Citations

Aug 12, 2014·Bioresource Technology·Mareike MonscheinBernd Nidetzky
Nov 18, 2015·Applied Microbiology and Biotechnology·Rossana LiguoriVincenza Faraco
Oct 12, 2013·Biotechnology Progress·Rosangela D P B PirotaCristiane S Farinas
Aug 6, 2015·Biotechnology for Biofuels·Zhongpeng GuoMichael Joseph O'Donohue
Mar 31, 2015·Applied Microbiology and Biotechnology·Daniel GomesMiguel Gama
Aug 23, 2018·Biotechnology and Bioengineering·Demi T DjajadiAnne S Meyer
Aug 26, 2020·Journal of Industrial Microbiology & Biotechnology·Heidi ØstbyAnikó Várnai
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michele MichelinJosé A Teixeira

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