Red and infrared laser therapy inhibits in vitro growth of major bacterial species that commonly colonize skin ulcers

Lasers in Medical Science
Natanael Teixeira Alves de SousaRinaldo Roberto de Jesus Guirro

Abstract

Low-level laser therapy (LLLT) is used in chronic wounds due to its healing effects. However, bacterial species may colonize these wounds and the optimal parameters for effective bacterial inhibition are not clear. The aim of this study was to analyze the effect of LLLT on bacterial growth in vitro. Bacterial strains including Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were suspended in saline solution at a concentration of 10(3) cells/ml and exposed to laser irradiation at wavelengths of 660, 830, and 904 nm at fluences of 0 (control), 3, 6, 12, 18, and 24 J/cm(2). An aliquot of the irradiated suspension was spread on the surface of petri plates and incubated at 37 °C for quantification of colony-forming unit after 24, 48, and 72 h. Laser irradiation inhibited the growth of S. aureus at all wavelengths and fluences higher than 12 J/cm(2), showing a strong correlation between increase in fluence and bacterial inhibition. However, for P. aeruginosa, LLLT inhibited growth at all wavelengths only at a fluence of 24 J/cm(2). E. coli had similar growth inhibition at a wavelength of 830 nm at fluences of 3, 6, 12, and 24 J/cm(2). At wavelengths of 660 and 904 nm, growth inhibition was only observed at fluence...Continue Reading

References

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May 9, 2015·Photomedicine and Laser Surgery·Natanael Teixeira Alves de SousaRinaldo Roberto de Jesus Guirro

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Citations

Feb 6, 2017·Journal of Photochemistry and Photobiology. B, Biology·Morena PetriniSimonetta D'Ercole
Jun 9, 2018·Photochemistry and Photobiology·Michael R HamblinVladimir Heiskanen
Oct 12, 2019·NPJ Biofilms and Microbiomes·Katia RupelSerena Zacchigna
Oct 10, 2019·Photobiomodulation, Photomedicine, and Laser Surgery·Ann LiebertMichael R Hamblin
Jun 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sara García-SalinasSilvia Irusta
Jul 22, 2019·Lasers in Medical Science·Lidija NemethKsenija Cankar
Feb 20, 2018·Frontiers in Microbiology·Peter J Gwynne, Maurice P Gallagher
Jan 25, 2021·Lasers in Medical Science·Adenilson de Souza da FonsecaFlavia de Paoli
Oct 9, 2020·NPJ Biofilms and Microbiomes·Katia RupelSerena Zacchigna
Feb 22, 2021·Drug Delivery and Translational Research·Pia KaiserMaike Windbergs

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