Red Blood Cell Metabolic Responses to Torpor and Arousal in the Hibernator Arctic Ground Squirrel

Journal of Proteome Research
Sarah GehrkeAngelo D'Alessandro

Abstract

Arctic ground squirrels provide a unique model to investigate metabolic responses to hibernation in mammals. During winter months these rodents are exposed to severe hypothermia, prolonged fasting, and hypoxemia. In the light of their role in oxygen transport/off-loading and owing to the absence of nuclei and organelles (and thus de novo protein synthesis capacity), mature red blood cells have evolved metabolic programs to counteract physiological or pathological hypoxemia. However, red blood cell metabolism in hibernation has not yet been investigated. Here we employed targeted and untargeted metabolomics approaches to investigate erythrocyte metabolism during entrance to torpor to arousal, with a high resolution of the intermediate time points. We report that torpor and arousal promote metabolism through glycolysis and pentose phosphate pathway, respectively, consistent with previous models of oxygen-dependent metabolic modulation in mature erythrocytes. Erythrocytes from hibernating squirrels showed up to 100-fold lower levels of biomarkers of reperfusion injury, such as the pro-inflammatory dicarboxylate succinate. Altered tryptophan metabolism during torpor was here correlated to the accumulation of potentially neurotoxic ...Continue Reading

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Citations

Oct 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Theresa L MontgomeryDimitry N Krementsov
Dec 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chao ChenDaniel A Hahn
Apr 6, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Reem AboushoushaYvonne M W Janssen-Heininger
May 18, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Valentina Lukinović, Kyle K Biggar

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