Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10

The Plant Journal : for Cell and Molecular Biology
Richard V EspleyAndrew C Allan

Abstract

Anthocyanin concentration is an important determinant of the colour of many fruits. In apple (Malus x domestica), centuries of breeding have produced numerous varieties in which levels of anthocyanin pigment vary widely and change in response to environmental and developmental stimuli. The apple fruit cortex is usually colourless, although germplasm does exist where the cortex is highly pigmented due to the accumulation of either anthocyanins or carotenoids. From studies in a diverse array of plant species, it is apparent that anthocyanin biosynthesis is controlled at the level of transcription. Here we report the transcript levels of the anthocyanin biosynthetic genes in a red-fleshed apple compared with a white-fleshed cultivar. We also describe an apple MYB transcription factor, MdMYB10, that is similar in sequence to known anthocyanin regulators in other species. We further show that this transcription factor can induce anthocyanin accumulation in both heterologous and homologous systems, generating pigmented patches in transient assays in tobacco leaves and highly pigmented apple plants following stable transformation with constitutively expressed MdMYB10. Efficient induction of anthocyanin biosynthesis in transient assays...Continue Reading

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Citations

Feb 3, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Julie GrahamSusan McCallum
Apr 27, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Susan McCallumJulie Graham
Dec 12, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J M BushakraS E Gardiner
Dec 17, 2010·Plant Cell Reports·Cesar Aza-GonzálezNeftalí Ochoa-Alejo
Apr 16, 2013·Plant Molecular Biology·Nigel E GapperJames J Giovannoni
Mar 3, 2012·Transgenic Research·Giorgio Gambino, Ivana Gribaudo
Jul 23, 2013·Trends in Plant Science·Laura Jaakola
Aug 30, 2008·Journal of Experimental Botany·Roosa A E LaitinenPaula Elomaa
Feb 1, 2011·Journal of Experimental Botany·Imène HichriVirginie Lauvergeat
Jun 22, 2012·Journal of Experimental Botany·Charles Ampomah-DwamenaAndrew C Allan
Aug 4, 2012·Journal of Experimental Botany·Rebecca A Henry-KirkAndrew C Allan
May 8, 2010·Plant & Cell Physiology·Kazuko YoshidaMasaaki Sakuta
May 20, 2008·Plant Physiology·Vladimir ShulaevRichard E Veilleux
May 12, 2010·Plant Physiology·Xue-Ren YinIan B Ferguson

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Datasets Mentioned

BETA
AF117268
ABB83827
ABB83828
CAB09230
NP176813
NM105308
NM121479
AB097924
AAF66727
CAD87010

Methods Mentioned

BETA
transgenic
PCR
transfection

Software Mentioned

Vector NTI
LightCycler
BLAST
Clustal W
MEGA

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