Red-emitting DPSB-based conjugated polymer nanoparticles with high two-photon brightness for cell membrane imaging

ACS Applied Materials & Interfaces
Peng LiuYong Cao

Abstract

New red-emitting conjugated polymers have been successfully synthesized by incorporating classical two-photon absorption (TPA) units, electron-rich units, and a small amount of electron-deficient units along the polymer backbones. Water-dispersible nanoparticles (NPs) based on these polymers were also fabricated for applications in two-photon excitation fluorescence imaging of cell membrane. Through optimization of the polymer/matrix mass ratio and the initial feed concentration of the polymer solution, a high quantum yield (QY) of 24% was achieved for the red-emitting NPs in water. TPA cross section and two-photon action cross section values of these polymers at 750 nm reached up to 1000 GM and 190 GM per repeat unit in aqueous media, 2.5 × 10(5) GM and 4.7 × 10(4) GM per NP, respectively. Furthermore, these NPs displayed excellent photostability and biocompatibility. Their applications as two-photon excitation fluorescence probes for cell membrane imaging have been demonstrated in three different cell lines with excellent imaging contrast. These results demonstrated that these polymer NPs hold great potentials as excellent two-photon excitation fluorescence probes in various biological applications.

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Citations

Jul 28, 2015·Chemical Society Reviews·Jingjing LiJun-Jie Zhu
Sep 24, 2015·ACS Applied Materials & Interfaces·Zhifang ChenYuyang Jiang
Jun 2, 2015·Physical Chemistry Chemical Physics : PCCP·Xinyuan ZhangShengping Ruan
Jul 1, 2016·Small·Xiaoding LouBen Zhong Tang
Jan 16, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Thais Fedatto AbelhaLea Ann Dailey
Dec 12, 2020·Soft Matter·Amir JangizehiSebastian Seiffert
Apr 8, 2020·ACS Applied Materials & Interfaces·Yibin ZhangHaiying Liu
Oct 20, 2018·Analytical Chemistry·Chamari S WijesooriyaEmily A Smith

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