Red fluorescent protein (DsRFP) optimization for Entamoeba histolytica expression

Experimental Parasitology
Cindy Jazmín Delgado-GalvánBernardo Franco

Abstract

Entamoeba histolytica genetic organization and genome structure is complex and under intense research. The genome is fully sequenced, and several tools have been developed for the molecular study of this organism. Nevertheless, good protein tracking tags that are easy to measure and image, like the fluorescent proteins are lacking. In this report, we codon-optimized the red fluorescent protein from the coral Discosoma striata (DsRFP) for its use in E. histolytica and demonstrated functionality in vivo. We envision that this protein can be widely used for the development of transcriptional reporter systems and protein-tagging applications.

Citations

Aug 29, 2019·PLoS Pathogens·Fátima Ramírez-MontielFelipe Padilla-Vaca
Aug 7, 2019·Protein and Peptide Letters·Jessica Tamayo-NuñezBernardo Franco

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