Red fluorescent protein variants with incorporated non-natural amino acid analogues

Protein Engineering, Design & Selection : PEDS
Ann GouldingSapna K Deo

Abstract

Fluorescent proteins are important tools in biotechnology applications and biosensing. DsRed, a red fluorescent protein, has expanded the colors of fluorescent proteins beyond the more commonly used green fluorescent protein. Many genetic modifications have been performed on DsRed to overcome some of its drawbacks. These primarily focused on overcoming the oligomerization detrimental to DsRed activity, and the parasitic green fluorescence caused by the immature chromophore. One such variant, DsRed-monomer, has minimal green fluorescence and no oligomerization. A few traditional mutagenesis studies have been done with DsRed and its mutants to shift the fluorescence wavelengths creating additions to the pallet of fluorescent protein colors. We have explored incorporation of non-natural amino acid analogues into DsRed-Monomer, obtaining variants with differing emission properties. In this work, two such analogues of tyrosine have been incorporated into DsRed-Monomer: 3-amino-l-tyrosine and 3-fluoro-l-tyrosine. Tyrosine analogues were chosen due to the role of tyrosine in the formation and structure of the protein's chromophore. The variants obtained in our study showed altered emission wavelengths and spectral characteristics. Our...Continue Reading

Citations

Dec 24, 2011·The Journal of Chemical Physics·Prem P ChapagainWilliam Castillo
Oct 15, 2013·Acta Biomaterialia·Jennifer E GagnerElliot L Chaikof
Dec 17, 2009·Journal of Computational Chemistry·Elsa Sanchez-GarciaWalter Thiel
Aug 19, 2016·Physical Chemistry Chemical Physics : PCCP·M Alaraby SalemAlex Brown
Nov 13, 2010·Current Opinion in Chemical Biology·Jeremiah A JohnsonDavid A Tirrell
Aug 21, 2021·Chembiochem : a European Journal of Chemical Biology·Matthew C T Hartman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Chembiochem : a European Journal of Chemical Biology
Lars MerkelNediljko Budisa
Applied Microbiology and Biotechnology
Annette Müller-Taubenberger, Kurt I Anderson
Proceedings of the National Academy of Sciences of the United States of America
Matthew D ShouldersRonald T Raines
Proceedings of the National Academy of Sciences of the United States of America
Christina WolschnerNediljko Budisa
© 2021 Meta ULC. All rights reserved