Red wine extract protects against oxidative-stress-induced endothelial senescence

Clinical Science
Ilse P G BotdenAnton J M Roks

Abstract

Red wine polyphenols may preserve endothelial function during aging. Endothelial cell senescence enhances age-related endothelial dysfunction. We investigated whether RWE (red wine extract) prevents oxidative-stress-induced senescence in HUVECs (human umbilical-vein endothelial cells). Senescence was induced by exposing HUVECs to tBHP (t-butylhydroperoxide), and quantified by senescence-associated β-galactosidase staining. RWE (0-50 μg/ml) concentration dependently decreased senescence by maximally 33±7.1%. RWE prevented the senescence-associated increase in p21 protein expression, inhibited tBHP-induced DNA damage of endothelial cells and induced relaxation of PCAs (porcine coronary arteries). Inhibition of SIRT1 (sirtuin 1) by sirtinol partially reversed the effect of RWE on tBHP-induced senescence, whereas both the NOS (nitric oxide synthase) inhibitor L-NMMA (NG-monomethyl-L-arginine) and the COX (cyclo-oxygenase) inhibitor indomethacin fully inhibited it. Furthermore, incubation of HUVECs with RWE increased eNOS (endothelial NOS) and COX-2 mRNA levels as well as phosphorylation of eNOS at Ser1177. RWE protects endothelial cells from tBHP-induced senescence. NO and COX-2, in addition to activation of SIRT1, play a critical ...Continue Reading

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Citations

Sep 13, 2015·Vascular Pharmacology·Francesca SantilliGiovanni Davì
Aug 21, 2013·Trends in Cardiovascular Medicine·Haiyan Wu, Anton J M Roks
Jan 30, 2013·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Peter Stenvinkel, Tobias E Larsson
Apr 2, 2013·Free Radical Biology & Medicine·Jae-woong HwangIrfan Rahman
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Jan 11, 2017·Applied Microbiology and Biotechnology·Sascha Sauer, Annabell Plauth
Apr 2, 2020·Free Radical Research·Jeongmin ParkHun Taeg Chung
Sep 2, 2017·Biomedit︠s︡inskai︠a︡ khimii︠a︡·M V MikhailovaA I Archakov
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Oct 16, 2015·Clinical Science·Paula K Bautista NiñoAnton J M Roks
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