Redefining Molecular Amphipathicity in Reversing the "Coffee-Ring Effect": Implications for Single Base Mutation Detection

Langmuir : the ACS Journal of Surfaces and Colloids
Chi HuangQuan Yuan

Abstract

The "coffee ring effect" is a natural phenomenon wherein sessile drops leave ring-shaped structures on the solid surfaces upon drying. It drives a nonuniform deposition of suspended compounds on the substrates, which adversely affects many processes, including surface-assisted biosensing and molecular self-assembly. In this study, we describe how the coffee ring effect can be eliminated by controlling the amphipathicity of the suspended compounds, for example, DNA modified with hydrophobic dye. Specifically, nuclease digestion of the hydrophilic DNA end converts the dye-labeled molecule into an amphipathic molecule (one with comparably weighted hydrophobic and hydrophilic ends) and reverses the coffee ring effect and results in a uniform disk-shaped feature deposition of the dye. The amphipathic product decreases the surface tension of the sessile drops and induces the Marangoni flow, which drives the uniform distribution of the amphipathic dye-labeled product in the drops. As a proof of concept, this strategy was used in a novel enzymatic amplification method for biosensing to eliminate the coffee ring effect on a nitrocellulose membrane and increase assay reliability and sensitivity. Importantly, the reported strategy for eli...Continue Reading

References

May 2, 2003·Analytical Chemistry·Yaxin JiangChunli Bai
Apr 6, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Jungho Park, Jooho Moon
Apr 23, 2008·Journal of the American Chemical Society·Yangjun Cai, Bi-min Zhang Newby
Jan 23, 2010·Journal of the American Chemical Society·Xiaolei ZuoKevin W Plaxco
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Joshua R TrantumFrederick R Haselton
Feb 23, 2012·Nature Chemistry·David Yu ZhangPeng Yin
Apr 25, 2013·Nature Communications·Wouter SempelsJan Vermant
Nov 14, 2013·Biosensors & Bioelectronics·Chi-Chang LinHsien-Chang Chang
Nov 22, 2013·Lab on a Chip·Joshua R TrantumFrederick R Haselton
Jan 2, 2014·Accounts of Chemical Research·Hao PeiChunhai Fan
Apr 2, 2014·Advanced Materials·Minxuan KuangYanlin Song
May 23, 2014·Accounts of Chemical Research·Yuanchen DongDongsheng Liu
Oct 4, 2014·Chemical Communications : Chem Comm·Huaguo XuHaojun Liang
Oct 8, 2014·Angewandte Chemie·Manos Anyfantakis, Damien Baigl
Oct 15, 2014·Analytical Chemistry·Feng LiX Chris Le
Dec 19, 2014·The Journal of Physical Chemistry. B·Venkateshwar Rao Dugyala, Madivala G Basavaraj
Apr 9, 2015·Nanoscale Research Letters·Jamie BensonKar Seng Teng
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Seyed-Fakhreddin TorabiYi Lu
Aug 4, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Manos Anyfantakis, Damien Baigl
Dec 8, 2015·ACS Applied Materials & Interfaces·Jiazhen SunYanlin Song
Apr 20, 2016·Scientific Reports·Yanan LiYanlin Song
May 24, 2016·Chemistry, an Asian Journal·Jue HouYanlin Song
Jun 23, 2016·Analytical Chemistry·Ruth Hernandez-PerezJose L Garcia-Cordero
Dec 31, 2016·Angewandte Chemie·Yuanchen DongDongsheng Liu
Jan 13, 2017·Angewandte Chemie·Xiangmeng QuChunhai Fan
Jun 1, 2017·Lab on a Chip·Jose L Garcia-Cordero, Z Hugh Fan
Jun 7, 2017·Journal of the American Chemical Society·Ruowen WangWeihong Tan
Jun 10, 2017·Chemical Reviews·Wenhu ZhouJuewen Liu

❮ Previous
Next ❯

Citations

Jul 31, 2020·Biosensors & Bioelectronics·Chi HuangXue-Feng Yu
Nov 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Byeong-Ui MoonTeodor Veres
Sep 4, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Fangfei YinXiaolei Zuo
Sep 16, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Qishen HuangPeter J Vikesland

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.