Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology

Genome Biology
Gaetan Burgio

Abstract

The generation of genetically modified alleles in mice using conventional transgenesis technologies is a long and inefficient process. A new study shows that the in situ delivery of CRISPR/Cas9 reagents into pregnant mice results in a high efficiency of editing, and enables the rapid generation of both simple and complex alleles.

Citations

Mar 21, 2019·Frontiers in Bioengineering and Biotechnology·Michael F EckerstorferFriedrich Waßmann
Oct 26, 2018·Physiological Reviews·Agustin Gonzalez-VicenteJeffrey L Garvin
Mar 12, 2020·Current Protocols in Mouse Biology·Almudena FernándezLluís Montoliu
Mar 28, 2020·Genes·Kenji Rowel Q LimToshifumi Yokota
Sep 17, 2019·Toxicologic Pathology·Kyathanahalli S JanardhanRamesh C Kovi
Feb 27, 2020·Antioxidants·Young Jae Lee
Jan 11, 2019·Disease Models & Mechanisms·Channabasavaiah B Gurumurthy, Kevin C Kent Lloyd
Jul 10, 2020·International Journal of Molecular Sciences·Young Jae Lee
Feb 12, 2021·Journal, Genetic Engineering & Biotechnology·Anuran GhoshPraphulla Chandra Shukla
Apr 30, 2021·Development·Ersin AkinciRichard I Sherwood

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Methods Mentioned

BETA
genetic modification
transgenic

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