Redefining MTBDRplus test results: what do indeterminate results actually mean?

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
Chaitali NikamCamilla Rodrigues

Abstract

Although line-probe assays (LPAs) are promising, little research has been conducted to elucidate the true nature of indeterminate LPA results or assess the ability of these assays to perform on a wide range of clinical samples. To evaluate the performance of the commercially available GenoType(®) MTBDRplus LPA against conventional BACTEC™ MGIT™ 960 culture and drug susceptibility testing (DST) among 308 pulmonary tuberculosis (PTB) and 32 extra-pulmonary TB samples. Invalid LPA results (defined as those with a missing Mycobacterium tuberculosis identification band) were obtained for 18 PTB samples, which were excluded from further analysis. The sensitivity and specificity of the MTBDRplus assay for multidrug-resistant TB, based upon the results obtained for the remaining 322 samples, was respectively 95.2% and 95.1%. Of 290 PTB samples, 40 (13.7%) were indeterminate on LPA (defined as the absence of both wild-type and corresponding mutation bands) for isoniazid (INH) and/or rifampicin (RMP), and were further evaluated by pyrosequencing (PSQ). Contrary to standard LPA interpretation, INH and RMP susceptibility were confirmed by both DST and PSQ in respectively 7.5% (3/40) and 27.5% (11/40) of indeterminate samples. PSQ was found...Continue Reading

Citations

Sep 3, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M L Pérez Del MolinoUNKNOWN Galician M. tuberculosis Resistance Group
Dec 12, 2019·BMC Infectious Diseases·Regina Bones BarcellosAfrânio Kritski
Aug 6, 2020·Tuberculosis and Respiratory Diseases·Binit Kumar SinghNaveet Wig
Oct 15, 2021·Annals of Clinical Microbiology and Antimicrobials·Mao YeEbrahim Kouhsari

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