Redefining Myeloid Cell Subsets in Murine Spleen

Frontiers in Immunology
Ying-ying HeyHelen C O'Neill

Abstract

Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis of phenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses, splenic dendritic cell (DC) subsets are now better characterized than other myeloid subsets. In order to identify and fully characterize a novel splenic subset termed "L-DC" in relation to other myeloid cells, it was necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DC were initially characterized as a CD11b(hi)CD11c(lo)MHCII(-)Ly6C(-)Ly6G(-) subset in murine spleen. Their expression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC by phenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to the classification of CD11b(hi)CD11c(lo)MHCII(-)Ly6C(lo)Ly6G(-) cells as monocytes expressing CX3CR1, CD43 and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishable from both Ly6C(lo) and Ly6C(hi) monocytes, and other DC subsets. L-DC were characterized as a clear subset of CD11b(hi)CD11c(lo)MHCII(-)Ly6C(-)Ly6G(-) cells, which are CD43(+), Siglec-F(-) and CD115(-). Changes in the ...Continue Reading

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Citations

Jan 30, 2018·Frontiers in Immunology·Adrian Y S LeeHeinrich Körner
Jun 26, 2018·The Journal of General Virology·Aroon SupramaniamLara J Herrero
Apr 11, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Christie ShortHelen C O'Neill
Jun 27, 2018·BMC Immunology·Sawang PetvisesHelen C O'Neill
Oct 12, 2018·PloS One·Pravin PeriasamyHelen C O'Neill
Aug 14, 2019·The Journal of Experimental Medicine·Ping-Hsien LeeNicholas P Restifo
Jan 13, 2017·BMC Immunology·Ying-Ying HeyHelen C O'Neill
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Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Benjamin N NelsonKaren L Wozniak
Feb 11, 2020·Frontiers in Endocrinology·Chloe HoggErin Greaves
Aug 1, 2020·Frontiers in Immunology·Elke M MuntjewerffGeert van den Bogaart
Jun 19, 2019·Journal of Cellular and Molecular Medicine·Ying-Ying HeyHelen C O'Neill
Jun 15, 2021·Frontiers in Medicine·William T Nash, Mark D Okusa
Sep 28, 2021·Cellular and Molecular Life Sciences : CMLS·Fuye YangHui Yao Lan

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
flow cytometry

Software Mentioned

FlowJo
BD FACSDiva

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