Redefining the significance of aneuploidy in the prognostic assessment of colorectal cancer

Laboratory Investigation; a Journal of Technical Methods and Pathology
R A RisquesM A Peinado

Abstract

The aberrant content of DNA, or aneuploidy, is a hallmark of tumor cells and may be associated with malignant potential. Based on the hypothesis that aneuploidy, as a form of genetic instability, results in an increased capability to generate cell heterogeneity, we investigated whether a comprehensive assessment of aneuploidy extent and degree might be a reliable indicator of tumor aggressiveness. DNA content was determined by flow cytometry in the infiltrating front of 131 paraffin-embedded primary colorectal carcinomas collected in a prospective design. Enrichment of tumor cells by sample microdissection resulted in neoplastic cell contents above 75%. An estimate of aneuploidy, the aneuploidy index (AI), was calculated as the tumor DNA content adjusted by the percentage of diploid and aneuploid cells in G0/G1. Thirty-nine tumors were diploid, 90 hyperdiploid, and 2 hypodiploid. The mean AI in aneuploid tumors was 1.20+/-0.17 and correlated with Dukes' stage and metastasis (p < 0.05). A high AI (receiver operating characteristic curve cutoff value greater than 1.14) predicted a poorer outcome in univariate (p = 0.004) and multivariate (p = 0.01) analyses. Based on these results, we postulate that aneuploidy is the molecular en...Continue Reading

References

Jul 1, 1992·The British Journal of Surgery·G T DeansR A Spence
Oct 15, 1988·International Journal of Cancer. Journal International Du Cancer·Y RemvikosB Dutrillaux
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M A Cifone, I J Fidler
Jan 1, 1994·Cold Spring Harbor Symposia on Quantitative Biology·M PeruchoE Stanbridge
Jan 1, 1993·Advances in Cancer Research·K C Cheng, L A Loeb
May 7, 1993·Science·S N ThibodeauD Schaid
Jan 1, 1995·Advances in Cancer Research·B Dutrillaux
Apr 10, 1997·Nature·C LengauerB Vogelstein
Oct 23, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R ArribasM A Peinado
Apr 1, 1998·Nature·D P CahillB Vogelstein
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·P DuesbergR Hehlmann
Jan 1, 1999·Nature·C LengauerB Vogelstein
May 20, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S TortolaG Capella
Aug 5, 1999·Journal of the National Cancer Institute·K C HallingS N Thibodeau
Apr 6, 2000·Journal of the National Cancer Institute·I González-GarcíaM A Peinado

❮ Previous
Next ❯

Citations

Jul 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Georgia BardiSverre Heim
Apr 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Khaled Refaat ZalataAshraf Shoma
Sep 18, 2007·Diseases of the Colon and Rectum·Sergio E A AraujoIvan Cecconello
Jun 3, 2006·Archives of Pathology & Laboratory Medicine·Philippe E Spiess, Bogdan Czerniak
Mar 31, 2005·Cancer Letters·Elisenda VendrellMiguel A Peinado

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.