Redesign the α/β fold to enhance the stability of mannanase Man23 from Bacillus subtilis

Applied Biochemistry and Biotechnology
Hai-Yan ZhouYong-Yao Wu

Abstract

In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high activity and stability at higher temperature and broader pH. This quartet variant was characterized by an almost threefold increased activity and a sevenfold increased stability compared to native mannanase Man23. Our results suggest that such work is safe to increase our target protein stability with no loss of activity.

References

Nov 1, 1978·Carbohydrate Research·B V McCleary
Nov 1, 1987·Biopolymers·W J Becktel, J A Schellman
Oct 1, 1996·Protein Science : a Publication of the Protein Society·R SternerK Kirschner
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·P ZávodszkyG A Petsko
Jan 23, 1999·Current Opinion in Structural Biology·R Jaenicke, G Böhm
Apr 25, 2000·Protein Engineering·S KumarR Nussinov
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Feb 28, 2002·Journal of Molecular Biology·John M JoernFrances H Arnold
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Feb 21, 2004·Plant Physiology·Sergei A FilichkinHiroyuki Nonogaki
Mar 30, 2004·Protein Engineering, Design & Selection : PEDS·Wen-Chen SuenAleksey Zaks
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Dec 19, 2007·Critical Reviews in Biotechnology·Samriti Dhawan, Jagdeep Kaur

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Citations

Feb 9, 2012·Applied Microbiology and Biotechnology·Prakram Singh ChauhanNaveen Gupta
May 2, 2015·Journal of the Science of Food and Agriculture·Haiyan ZhouYongyao Wu
May 20, 2016·Applied Biochemistry and Biotechnology·Haiyan ZhouYongyao Wu

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