PMID: 11604457Oct 18, 2001Paper

Redifferentiation of bacteria isolated from Lotus japonicus root nodules colonized by Rhizobium sp. NGR234

Journal of Experimental Botany
J MüllerT Boller

Abstract

In most studies concerning legume root nodules, the question to what extent the nodule-borne bacteroids survive nodule senescence has not been properly addressed. At present, there is no "model system" to study these aspects in detail. Such a system with Lotus japonicus and the broad host range Rhizobium sp. NGR234 has been developed. L. japonicus L. cv. Gifu was inoculated with Rhizobium sp. NGR234 and grown over a 12 week time period. The first nodules could be harvested after 3 weeks. Nodulation reached a plateau after 11 weeks with a mean of 64 nodules having a biomass of nearly 100 mg FW per plant. Nodules were harvested and homogenized at different stages of plant development. Microscopic inspection of the extracts revealed that, typically, nodules contained c. 15x10(9) bacteroids g(-1) FW, and that about 60% of the bacteroids were viable as judged by vital staining. When aliquots of the extracts were plated on selective media, a substantial number of "colony-forming units" was observed in all cases, indicating that a considerable fraction of the bacteroids had the potential to redifferentiate into growing bacteria. In nodules from the early developmental stages, the fraction of total bacteroids yielding CFUs amounted to ...Continue Reading

References

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Apr 3, 1999·Molecular Plant-microbe Interactions : MPMI·S G Pueppke, W J Broughton
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Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Michael K. Udvardi, David A. Day

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Citations

Jun 29, 2013·Genome Biology·Thomas R TurnerPhilip S Poole
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter MergaertEva Kondorosi
Jun 2, 2009·Applied and Environmental Microbiology·J L SachsE L Simms
Mar 30, 2010·Journal of Evolutionary Biology·J L SachsE L Simms
Oct 22, 2013·World Journal of Microbiology & Biotechnology·Osei Yaw Ampomah, John Beck Jensen
Mar 17, 2004·Journal of Plant Physiology·Christophe FargeixFrançois Widmer

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